PDBsum entry 4j89

Fluorescent protein

PDB id: 4j89

Contents

Protein chains

229 a.a.

Ligands

SO4 ×5

EDO ×23

TRS

Waters ×283

PDB id:

4j89

Name:

Fluorescent protein

Title:

Different photochemical events of a genetically encoded aryl azide define and modulate gfp fluorescence

Structure:

Green fluorescent protein. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Aequorea victoria. Jellyfish. Organism_taxid: 6100. Gene: gfp. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.10Å R-factor: 0.185 R-free: 0.242

Authors:

S.C.Reddington,D.D.Jones,P.J.Rizkallah,E.M.Tippmann

Key ref:

S.C.Reddington et al. (2013). Different photochemical events of a genetically encoded phenyl azide define and modulate GFP fluorescence. Angew Chem Int Ed Engl, 52, 5974-5977. PubMed id: 23620472 DOI: 10.1002/anie.201301490

Date:

14-Feb-13 Release date: 26-Jun-13

Protein chains

P42212  (GFP_AEQVI) -  Green fluorescent protein from Aequorea victoria

Seq: 238 a.a.

Struc: 229 a.a.*

* PDB and UniProt seqs differ at 13 residue positions (black crosses)

DOI no: 10.1002/anie.201301490

Angew Chem Int Ed Engl 52:5974-5977 (2013)

PubMed id: 23620472

Different photochemical events of a genetically encoded phenyl azide define and modulate GFP fluorescence.

S.C.Reddington, P.J.Rizkallah, P.D.Watson, R.Pearson, E.M.Tippmann, D.D.Jones.

ABSTRACT

Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed.