PDBsum entry 4j75

Ligase

PDB id: 4j75

Contents

Protein chains

374 a.a.

Ligands

TYM ×2

GOL ×2

Waters ×213

PDB id:

4j75

Name:

Ligase

Title:

Crystal structure of a parasite tRNA synthetase, product-bound

Structure:

Tryptophanyl-tRNA synthetase. Chain: a, b. Fragment: unp residues 229-632. Synonym: tryptophan--tRNA ligase. Engineered: yes

Source:

Plasmodium falciparum. Organism_taxid: 36329. Strain: 3d7. Gene: pf13_0205. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.40Å R-factor: 0.194 R-free: 0.223

Authors:

C.Y.Koh,J.E.Kim,C.L.M.J.Verlinde,W.G.J.Hol

Key ref:

C.Y.Koh et al. (2013). Crystal structures of Plasmodium falciparum cytosolic tryptophanyl-tRNA synthetase and its potential as a target for structure-guided drug design. Mol Biochem Parasitol, 189, 26-32. PubMed id: 23665145 DOI: 10.1016/j.molbiopara.2013.04.007

Date:

12-Feb-13 Release date: 22-May-13

Protein chains

Q8IDW3  (Q8IDW3_PLAF7) -  tryptophan--tRNA ligase from Plasmodium falciparum (isolate 3D7)

Seq: 632 a.a.

Struc: 374 a.a.

Enzyme reactions

• Enzyme class: E.C.6.1.1.2 - tryptophan--tRNA ligase.
• Reaction: tRNA(Trp) + L-tryptophan + ATP = L-tryptophyl-tRNA(Trp) + AMP + diphosphate + H⁺

tRNA(Trp) + L-tryptophan + ATP = L-tryptophyl-tRNA(Trp) (Bound ligand (Het Group name = TYM) matches with 62.16% similarity) + AMP + diphosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.molbiopara.2013.04.007

Mol Biochem Parasitol 189:26-32 (2013)

PubMed id: 23665145

Crystal structures of Plasmodium falciparum cytosolic tryptophanyl-tRNA synthetase and its potential as a target for structure-guided drug design.

C.Y.Koh, J.E.Kim, A.J.Napoli, C.L.Verlinde, E.Fan, F.S.Buckner, W.C.Van Voorhis, W.G.Hol.

ABSTRACT

No abstract given.