PDBsum entry 4j4h

Oxidoreductase

PDB id

4j4h

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Contents

			Protein chains

					259 a.a.

			Ligands

					NAI ×4


					1J1 ×4

			Metals

					_MG ×4


					_NA ×4

			Waters ×558

PDB id:

4j4h

Links

Name:

Oxidoreductase

Title:

Pyld in complex with pyrroline-carboxy-lysine and nadh

Structure:

Pyld. Chain: a, b, c, d. Engineered: yes

Source:

Methanosarcina barkeri. Organism_taxid: 269797. Strain: fusaro. Gene: mbar_a0835. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.80Å

R-factor:

0.181

R-free:

0.218

Authors:

F.Quitterer,P.Beck,A.Bacher,M.Groll

Key ref:

F.Quitterer et al. (2013). Structure and reaction mechanism of pyrrolysine synthase (PylD). Angew Chem Int Ed Engl, 52, 7033-7037. PubMed id: 23720358 DOI: 10.1002/anie.201301164

Date:

06-Feb-13

Release date:

12-Jun-13

PROCHECK

	Headers

	References

Protein chains

Q46E80 (PYLD_METBF) - Pyrrolysine synthase from Methanosarcina barkeri (strain Fusaro / DSM 804)

Seq: Struc:					263 a.a. 259 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.1.4.1.- - ?????

[IntEnz] [ExPASy] [KEGG] [BRENDA]

DOI no: 10.1002/anie.201301164	Angew Chem Int Ed Engl 52:7033-7037 (2013)
PubMed id: 23720358

Structure and reaction mechanism of pyrrolysine synthase (PylD).
F.Quitterer, P.Beck, A.Bacher, M.Groll.

ABSTRACT

The final step in the biosynthesis of the 22nd genetically encoded amino acid, pyrrolysine, is catalyzed by PylD, a structurally and mechanistically unique dehydrogenase. This catalyzed reaction includes an induced-fit mechanism achieved by major structural rearrangements of the N-terminal helix upon substrate binding. Different steps of the reaction trajectory are visualized by complex structures of PylD with substrate and product.