PDBsum entry 4j2k

Hydrolase inhibitor

PDB id: 4j2k

Contents

Protein chains

168 a.a.

Ligands

IMD ×2

GOL ×2

Waters ×229

PDB id:

4j2k

Name:

Hydrolase inhibitor

Title:

Crystal structure of a plant trypsin inhibitor ecti

Structure:

Trypsin inhibitor. Chain: a, b. Synonym: ecti

Source:

Enterolobium contortisiliquum. Pacara earpod tree. Organism_taxid: 55671

Resolution:

1.75Å R-factor: 0.184 R-free: 0.237

Authors:

D.Zhou,A.Wlodawer

Key ref:

D.Zhou et al. (2013). Crystal structures of a plant trypsin inhibitor from Enterolobium contortisiliquum (EcTI) and of its complex with bovine trypsin. Plos One, 8, e62252. PubMed id: 23626794 DOI: 10.1371/journal.pone.0062252

Date:

04-Feb-13 Release date: 08-May-13

Protein chains

P86451  (ITRY_ENTCO) -  Trypsin inhibitor from Enterolobium contortisiliquum

Seq: 174 a.a.

Struc: 168 a.a.*

* PDB and UniProt seqs differ at 20 residue positions (black crosses)

DOI no: 10.1371/journal.pone.0062252

Plos One 8:e62252 (2013)

PubMed id: 23626794

Crystal structures of a plant trypsin inhibitor from Enterolobium contortisiliquum (EcTI) and of its complex with bovine trypsin.

D.Zhou, Y.A.Lobo, I.F.Batista, R.Marques-Porto, A.Gustchina, M.L.Oliva, A.Wlodawer.

ABSTRACT

A serine protease inhibitor from Enterolobium contortisiliquum (EcTI) belongs to the Kunitz family of plant inhibitors, common in plant seeds. It was shown that EcTI inhibits the invasion of gastric cancer cells through alterations in integrin-dependent cell signaling pathway. We determined high-resolution crystal structures of free EcTI (at 1.75 Å) and complexed with bovine trypsin (at 2 Å). High quality of the resulting electron density maps and the redundancy of structural information indicated that the sequence of the crystallized isoform contained 176 residues and differed from the one published previously. The structure of the complex confirmed the standard inhibitory mechanism in which the reactive loop of the inhibitor is docked into trypsin active site with the side chains of Arg64 and Ile65 occupying the S1 and S1' pockets, respectively. The overall conformation of the reactive loop undergoes only minor adjustments upon binding to trypsin. Larger deviations are seen in the vicinity of Arg64, driven by the needs to satisfy specificity requirements. A comparison of the EcTI-trypsin complex with the complexes of related Kunitz inhibitors has shown that rigid body rotation of the inhibitors by as much as 15° is required for accurate juxtaposition of the reactive loop with the active site while preserving its conformation. Modeling of the putative complexes of EcTI with several serine proteases and a comparison with equivalent models for other Kunitz inhibitors elucidated the structural basis for the fine differences in their specificity, providing tools that might allow modification of their potency towards the individual enzymes.