PDBsum entry 4j1v

Peptide binding protein/viral protein

PDB id: 4j1v

Contents

Protein chains

166 a.a.

15 a.a.

Ligands

ALA-LEU-PRO-ALA-TRP-ALA-ARG-PRO-ASP ×2

Metals

_ZN ×2

Waters ×386

PDB id:

4j1v

Name:

Peptide binding protein/viral protein

Title:

Functional and structural studies of mobkl1b, a salvador/warts/hippo tumor suppressor pathway, in hcv replication

Structure:

Mob kinase activator 1a. Chain: a, c. Synonym: mob1 alpha, mob1a, mob1 homolog 1b, mps one binder kinase activator-like 1b. Engineered: yes. Ns5a domain ii peptide. Chain: e, g, f, h. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mob1a, c2orf6, mob4b, mobk1b, mobkl1b. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Hepatitis c virus. Hcv.

Resolution:

1.95Å R-factor: 0.196 R-free: 0.225

Authors:

H.-Y.Chung,M.Gu,C.M.Rice

Key ref:

H.Y.Chung et al. (2014). Seed sequence-matched controls reveal limitations of small interfering RNA knockdown in functional and structural studies of hepatitis C virus NS5A-MOBKL1B interaction. J Virol, 88, 11022-11033. PubMed id: 25031347 DOI: 10.1128/JVI.01582-14

Date:

02-Feb-13 Release date: 06-Aug-14

Protein chains

Q9H8S9  (MOB1A_HUMAN) -  MOB kinase activator 1A from Homo sapiens

Seq: 216 a.a.

Struc: 166 a.a.

Protein chains

Q99IB8  (POLG_HCVJF) -  Genome polyprotein from Hepatitis C virus genotype 2a (isolate JFH-1)

Seq: 3033 a.a.

Struc: 15 a.a.

Enzyme reactions

• Enzyme class 2: Chains G, H: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 3: Chains G, H: E.C.3.4.21.98 - hepacivirin.
• Reaction: Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
• Enzyme class 4: Chains G, H: E.C.3.4.22.- - ?????
• Enzyme class 5: Chains G, H: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺
• Enzyme class 6: Chains G, H: E.C.3.6.4.13 - Rna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1128/JVI.01582-14

J Virol 88:11022-11033 (2014)

PubMed id: 25031347

Seed sequence-matched controls reveal limitations of small interfering RNA knockdown in functional and structural studies of hepatitis C virus NS5A-MOBKL1B interaction.

H.Y.Chung, M.Gu, E.Buehler, M.R.MacDonald, C.M.Rice.

ABSTRACT

No abstract given.