PDBsum entry 4j1m

Hydrolase

PDB id: 4j1m

Contents

Protein chain

129 a.a.

Ligands

THP

Metals

_CA

Waters ×113

PDB id:

4j1m

Name:

Hydrolase

Title:

Crystal structure of staphylococcal nuclease variant delta+phs r105e at cryogenic temperature

Structure:

Thermonuclease. Chain: a. Fragment: nuclease a (unp residues 83-231). Synonym: tnase, micrococcal nuclease, staphylococcal nuclease. Engineered: yes. Mutation: yes

Source:

Staphylococcus aureus. Organism_taxid: 1280. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.65Å R-factor: 0.181 R-free: 0.214

Authors:

P.C.Bell-Upp,J.L.Schlessman,B.Garcia-Moreno E.

Key ref:

P.C.Bell-Upp et al. Crystal structure of staphylococcal nuclease variant delta+phs r105e at cryogenic temperature. To be published, .

Date:

01-Feb-13 Release date: 20-Feb-13

Protein chain

P00644  (NUC_STAAU) -  Thermonuclease from Staphylococcus aureus

Seq: 231 a.a.

Struc: 129 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.31.1 - micrococcal nuclease.
• Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.