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PDBsum entry 4hpm
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Transcription
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PDB id
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4hpm
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Contents |
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118 a.a.
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81 a.a.
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107 a.a.
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82 a.a.
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PDB id:
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| Name: |
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Transcription
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Title:
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Pcgf1 ub fold (rawul)/bcorl1 pufd complex
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Structure:
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Bcl-6 corepressor-like protein 1. Chain: a, c. Fragment: unp residues 1594-1711. Synonym: bcor-l1, bcor-like protein 1. Engineered: yes. Polycomb group ring finger protein 1. Chain: b, d. Fragment: unp residues 167-255. Synonym: nervous system polycomb-1, nspc1, ring finger protein 68,
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: bcorl1. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: nspc1, pcgf1, rnf68.
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Resolution:
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1.85Å
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R-factor:
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0.202
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R-free:
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0.247
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Authors:
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S.E.Junco,R.Wang,J.Gaipa,A.B.Taylor,M.D.Gearhart,V.J.Bardwell, P.J.Hart,C.A.Kim
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Key ref:
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S.E.Junco
et al.
(2013).
Structure of the polycomb group protein PCGF1 in complex with BCOR reveals basis for binding selectivity of PCGF homologs.
Structure,
21,
665-671.
PubMed id:
DOI:
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Date:
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24-Oct-12
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Release date:
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01-May-13
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PROCHECK
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Headers
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References
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Q5H9F3
(BCORL_HUMAN) -
BCL-6 corepressor-like protein 1 from Homo sapiens
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Seq:
Struc:
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1785 a.a.
118 a.a.
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Q9BSM1
(PCGF1_HUMAN) -
Polycomb group RING finger protein 1 from Homo sapiens
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Seq:
Struc:
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259 a.a.
81 a.a.*
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DOI no:
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Structure
21:665-671
(2013)
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PubMed id:
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Structure of the polycomb group protein PCGF1 in complex with BCOR reveals basis for binding selectivity of PCGF homologs.
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S.E.Junco,
R.Wang,
J.C.Gaipa,
A.B.Taylor,
V.Schirf,
M.D.Gearhart,
V.J.Bardwell,
B.Demeler,
P.J.Hart,
C.A.Kim.
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ABSTRACT
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Polycomb-group RING finger homologs (PCGF1, PCGF2, PCGF3, PCGF4, PCGF5, and
PCGF6) are critical components in the assembly of distinct Polycomb repression
complex 1 (PRC1)-related complexes. Here, we identify a protein interaction
domain in BCL6 corepressor, BCOR, which binds the RING finger- and
WD40-associated ubiquitin-like (RAWUL) domain of PCGF1 (NSPC1) and PCGF3 but not
of PCGF2 (MEL18) or PCGF4 (BMI1). Because of the selective binding, we have
named this domain PCGF Ub-like fold discriminator (PUFD). The structure of BCOR
PUFD bound to PCGF1 reveals that (1) PUFD binds to the same surfaces as observed
for a different Polycomb group RAWUL domain and (2) the ability of PUFD to
discriminate among RAWULs stems from the identity of specific residues within
these interaction surfaces. These data show the molecular basis for determining
the binding preference for a PCGF homolog, which ultimately helps determine the
identity of the larger PRC1-like assembly.
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