PDBsum entry 4fvq

Transferase

PDB id: 4fvq

Contents

Protein chain

274 a.a.

Ligands

ATP

ACT ×2

Metals

_MG

Waters ×147

PDB id:

4fvq

Name:

Transferase

Title:

Crystal structure of the jak2 pseudokinase domain (mg-atp-bound form)

Structure:

Tyrosine-protein kinase jak2. Chain: a. Fragment: jak2 pseudokinase domain, unp residues 536-812. Synonym: janus kinase 2, jak-2. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: jak2. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9.

Resolution:

1.75Å R-factor: 0.176 R-free: 0.199

Authors:

R.M.Bandaranayake,S.R.Hubbard

Key ref:

R.M.Bandaranayake et al. (2012). Crystal structures of the JAK2 pseudokinase domain and the pathogenic mutant V617F. Nat Struct Biol, 19, 754-759. PubMed id: 22820988

Date:

29-Jun-12 Release date: 25-Jul-12

Protein chain

O60674  (JAK2_HUMAN) -  Tyrosine-protein kinase JAK2 from Homo sapiens

Seq: 1132 a.a.

Struc: 274 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.10.2 - non-specific protein-tyrosine kinase.
• Reaction: L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

L-tyrosyl-[protein] (Bound ligand (Het Group name = ATP) corresponds exactly) + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Nat Struct Biol 19:754-759 (2012)

PubMed id: 22820988

Crystal structures of the JAK2 pseudokinase domain and the pathogenic mutant V617F.

R.M.Bandaranayake, D.Ungureanu, Y.Shan, D.E.Shaw, O.Silvennoinen, S.R.Hubbard.

ABSTRACT

No abstract given.