PDBsum entry 4flc

Lyase

PDB id: 4flc

Contents

Protein chain

460 a.a.

Waters ×144

PDB id:

4flc

Name:

Lyase

Title:

Structural and biochemical characterization of human adenylosuccinate lyase (adsl) and the r303c adsl deficiency associated mutation

Structure:

Adenylosuccinate lyase. Chain: a, b, c, d. Synonym: asl, adenylosuccinase, asase. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: adsl, amps. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.60Å R-factor: 0.234 R-free: 0.298

Authors:

M.K.Deaton,S.P.Ray,G.C.Capodagli,L.A.F.Calkins,L.Sawle,K.Ghosh, D.Patterson,S.D.Pegan

Key ref:

S.P.Ray et al. (2012). Structural and biochemical characterization of human adenylosuccinate lyase (ADSL) and the R303C ADSL deficiency-associated mutation. Biochemistry, 51, 6701-6713. PubMed id: 22812634

Date:

14-Jun-12 Release date: 01-Aug-12

Protein chains

P30566  (PUR8_HUMAN) -  Adenylosuccinate lyase from Homo sapiens

Seq: 484 a.a.

Struc: 460 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.4.3.2.2 - adenylosuccinate lyase.
• Pathway: Purine Biosynthesis (late stages)
• Reaction:
  1. N₆-(1,2-dicarboxyethyl)-AMP = fumarate + AMP
  2. (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4- carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4- carboxamide + fumarate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Biochemistry 51:6701-6713 (2012)

PubMed id: 22812634

Structural and biochemical characterization of human adenylosuccinate lyase (ADSL) and the R303C ADSL deficiency-associated mutation.

S.P.Ray, M.K.Deaton, G.C.Capodagli, L.A.Calkins, L.Sawle, K.Ghosh, D.Patterson, S.D.Pegan.

ABSTRACT

No abstract given.