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PDBsum entry 4f8c
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protein ligands Protein-protein interface(s) links
Cell cycle/protein binding PDB id
4f8c

 

 

 

 

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Contents
Protein chains
249 a.a.
74 a.a.
Ligands
EDO ×4
Waters ×318
PDB id:
4f8c
Name: Cell cycle/protein binding
Title: Structure of the cif:nedd8 complex - yersinia pseudotuberculosis cycle inhibiting factor in complex with human nedd8
Structure: Cycle inhibiting factor. Chain: a, c. Fragment: effector domain, unp residues 33-288. Engineered: yes. Mutation: yes. Nedd8. Chain: b, d. Synonym: neddylin, neural precursor cell expressed developmentally down-regulated protein 8, nedd-8, ubiquitin-like protein nedd8.
Source: Yersinia pseudotuberculosis. Organism_taxid: 502800. Strain: ypiii. Gene: ypk_1971. Expressed in: escherichia coli. Expression_system_taxid: 562. Homo sapiens. Human. Organism_taxid: 9606.
Resolution:
1.95Å     R-factor:   0.189     R-free:   0.235
Authors: A.Crow,M.Banfield
Key ref: A.Crow et al. The molecular basis of nedd8 deamidation by the bacte effector protein cif. Proc natl acad sci usa, .
Date:
17-May-12     Release date:   20-Jun-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
A0A0H3B1Q8  (CIF_YERPY) -  Protein-glutamine deamidase Cif from Yersinia pseudotuberculosis serotype O:3 (strain YPIII)
Seq:
Struc: 		290 a.a.
249 a.a.*
Protein chains
Pfam   ArchSchema ?
Q15843  (NEDD8_HUMAN) -  NEDD8 from Homo sapiens
Seq:
Struc: 		81 a.a.
74 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: Chains A, C: E.C.3.5.1.44  - protein-glutamine glutaminase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: L-glutaminyl-[protein] + H2O = L-glutamyl-[protein] + NH4+
Protein L-glutamine
 + H(2)O
 = protein L-glutamate
 + NH(3)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

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