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PDBsum entry 4f8b
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Oxidoreductase
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PDB id
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4f8b
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PDB id:
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| Name: |
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Oxidoreductase
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Title:
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Crystal structure of the covalent thioimide intermediate of unimodular nitrile reductase quef
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Structure:
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NADPH-dependent 7-cyano-7-deazaguanine reductase. Chain: a, b, c, d, e. Synonym: quef, 7-cyano-7-carbaguanine reductase, NADPH-dependent nitrile oxidoreductase, preq(0) reductase. Engineered: yes
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Source:
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Bacillus subtilis subsp. Subtilis. Organism_taxid: 224308. Strain: 168. Gene: bsu13750, quef, ykvm. Expressed in: escherichia coli. Expression_system_taxid: 469008.
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Resolution:
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2.50Å
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R-factor:
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0.193
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R-free:
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0.272
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Authors:
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B.Stec,M.A.Swairjo
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Key ref:
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V.M.Chikwana
et al.
(2012).
Structural basis of biological nitrile reduction.
J Biol Chem,
287,
30560-30570.
PubMed id:
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Date:
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17-May-12
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Release date:
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11-Jul-12
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PROCHECK
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Headers
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References
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O31678
(QUEF_BACSU) -
NADPH-dependent 7-cyano-7-deazaguanine reductase from Bacillus subtilis (strain 168)
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Seq:
Struc:
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165 a.a.
139 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Enzyme class:
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E.C.1.7.1.13
- preQ1 synthase.
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Reaction:
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7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-deazaguanine + 2 NADPH + 3 H+
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7-aminomethyl-7-carbaguanine
Bound ligand (Het Group name = )
corresponds exactly
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+
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2
×
NADP(+)
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=
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7-cyano-7-deazaguanine
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+
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2
×
NADPH
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+
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3
×
H(+)
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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J Biol Chem
287:30560-30570
(2012)
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PubMed id:
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Structural basis of biological nitrile reduction.
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V.M.Chikwana,
B.Stec,
B.W.Lee,
V.de Crécy-Lagard,
D.Iwata-Reuyl,
M.A.Swairjo.
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ABSTRACT
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