PDBsum entry 4f7x

Hydrolase

PDB id: 4f7x

Contents

Protein chain

129 a.a.

Ligands

THP

Metals

_CA

Waters ×123

PDB id:

4f7x

Name:

Hydrolase

Title:

Crystal structure of staphylococcal nuclease variant delta+phs i92a/l25a at cryogenic temperature

Structure:

Thermonuclease. Chain: a. Fragment: nuclease a (unp residues 83-231). Synonym: tnase, micrococcal nuclease, staphylococcal nuclease. Engineered: yes. Mutation: yes

Source:

Staphylococcus aureus. Organism_taxid: 1280. Gene: nuc. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.70Å R-factor: 0.165 R-free: 0.197

Authors:

J.A.Caro,I.Clark,J.L.Schlessman,A.Heroux,B.Garcia-Moreno E.

Key ref:

J.A.Caro et al. Pressure effects in proteins. To be published, .

Date:

16-May-12 Release date: 13-Jun-12

Protein chain

P00644  (NUC_STAAU) -  Thermonuclease from Staphylococcus aureus

Seq: 231 a.a.

Struc: 129 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.31.1 - micrococcal nuclease.
• Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.