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PDBsum entry 4exm
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Toxin, hydrolase
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PDB id
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4exm
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PDB id:
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| Name: |
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Toxin, hydrolase
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Title:
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The crystal structure of an engineered phage lysin containing the binding domain of pesticin and the killing domain of t4-lysozyme
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Structure:
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Pesticin, lysozyme chimera. Chain: a, b, c, d. Fragment: see remark 999. Synonym: endolysin, lysis protein, muramidase. Engineered: yes. Mutation: yes
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Source:
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Yersinia pestis, enterobacteria phage t4. Organism_taxid: 632, 10665. Gene: pst, yp_ppcp06, yppcp1.05c, e. Expressed in: escherichia coli. Expression_system_taxid: 562
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Resolution:
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2.60Å
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R-factor:
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0.201
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R-free:
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0.251
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Authors:
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N.Seddiki,N.Noinaj,J.W.Fairman,P.Lukacik,T.J.Barnard,S.K.Buchanan
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Key ref:
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P.Lukacik
et al.
(2012).
Structural engineering of a phage lysin that targets gram-negative pathogens.
Proc Natl Acad Sci U S A,
109,
9857-9862.
PubMed id:
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Date:
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30-Apr-12
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Release date:
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20-Jun-12
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PROCHECK
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Headers
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References
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P00720
(ENLYS_BPT4) -
Endolysin from Enterobacteria phage T4
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Seq:
Struc:
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164 a.a.
291 a.a.*
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Q57159
(Q57159_YERPE) -
Pesticin from Yersinia pestis
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Seq:
Struc:
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357 a.a.
291 a.a.*
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Enzyme class:
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Chains A, B, C, D:
E.C.3.2.1.17
- lysozyme.
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Reaction:
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Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.
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Proc Natl Acad Sci U S A
109:9857-9862
(2012)
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PubMed id:
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Structural engineering of a phage lysin that targets gram-negative pathogens.
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P.Lukacik,
T.J.Barnard,
P.W.Keller,
K.S.Chaturvedi,
N.Seddiki,
J.W.Fairman,
N.Noinaj,
T.L.Kirby,
J.P.Henderson,
A.C.Steven,
B.J.Hinnebusch,
S.K.Buchanan.
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ABSTRACT
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