PDBsum entry 4ex4

Transferase

PDB id: 4ex4

Contents

Protein chains

684 a.a.

721 a.a.

Ligands

EDO

Metals

_MG ×6

Waters ×866

PDB id:

4ex4

Name:

Transferase

Title:

The structure of glcb from mycobacterium leprae

Structure:

Malate synthase g. Chain: a, b. Engineered: yes

Source:

Mycobacterium leprae. Organism_taxid: 561304. Strain: br4923. Gene: glcb, ml2069, mlbr02069, mlcb1788.27. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.10Å R-factor: 0.183 R-free: 0.234

Authors:

Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

M.C.Clifton et al. The structure of glcb from mycobacterium leprae. To be published, .

Date:

29-Apr-12 Release date: 23-May-12

Protein chain

B8ZSN3  (MASZ_MYCLB) -  Malate synthase G from Mycobacterium leprae (strain Br4923)

Seq: 731 a.a.

Struc: 684 a.a.*

Protein chain

B8ZSN3  (MASZ_MYCLB) -  Malate synthase G from Mycobacterium leprae (strain Br4923)

Seq: 731 a.a.

Struc: 721 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.2.3.3.9 - malate synthase.
• Pathway: Glyoxylate Cycle
• Reaction: glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H⁺

glyoxylate + acetyl-CoA (Bound ligand (Het Group name = EDO) matches with 80.00% similarity) + H2O = (S)-malate + CoA + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site