PDBsum entry 4epb

Hydrolase

PDB id: 4epb

Contents

Protein chains

566 a.a.

101 a.a.

100 a.a.

Metals

_NI ×2

Waters ×652

PDB id:

4epb

Name:

Hydrolase

Title:

Final urease structure for radiation damage experiment at 100 k

Structure:

Urease subunit alpha. Chain: c. Synonym: urea amidohydrolase subunit alpha. Engineered: yes. Urease subunit beta. Chain: b. Synonym: urea amidohydrolase subunit beta. Engineered: yes. Urease subunit gamma.

Source:

Enterobacter aerogenes. Organism_taxid: 548. Gene: urec. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: ureb. Gene: urea. Expression_system_taxid: 562

Resolution:

1.75Å R-factor: 0.173 R-free: 0.197

Authors:

M.Warkentin,R.Badeau,J.B.Hopkins,R.E.Thorne

Key ref:

M.Warkentin et al. (2012). Spatial distribution of radiation damage to crystalline proteins at 25-300 K. Acta Crystallogr D Biol Crystallogr, 68, 1108-1117. PubMed id: 22948911

Date:

17-Apr-12 Release date: 29-Aug-12

Protein chain

P18314  (URE1_KLEAE) -  Urease subunit alpha from Klebsiella aerogenes

Seq: 567 a.a.

Struc: 566 a.a.*

Protein chain

P18315  (URE2_KLEAE) -  Urease subunit beta from Klebsiella aerogenes

Seq: 106 a.a.

Struc: 101 a.a.

Protein chain

P18316  (URE3_KLEAE) -  Urease subunit gamma from Klebsiella aerogenes

Seq: 100 a.a.

Struc: 100 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains C, B, A: E.C.3.5.1.5 - urease.
• Reaction: urea + 2 H2O + H⁺ = hydrogencarbonate + 2 NH4⁺
• Cofactor: Ni(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Acta Crystallogr D Biol Crystallogr 68:1108-1117 (2012)

PubMed id: 22948911

Spatial distribution of radiation damage to crystalline proteins at 25-300 K.

M.Warkentin, R.Badeau, J.B.Hopkins, R.E.Thorne.

ABSTRACT

No abstract given.