PDBsum entry 4egc

Transcription/hydrolase

PDB id

4egc

Loading ...

Contents

			Protein chains

					539 a.a.


					255 a.a.

			Ligands

					GLC-GLC

			Metals

					_MG

			Waters ×812

PDB id:

4egc

Links

Name:

Transcription/hydrolase

Title:

Crystal structure of mbp-fused human six1 bound to human eya2 eya domain

Structure:

Maltose-binding periplasmic protein, homeobox protein six1 chimera. Chain: a. Fragment: see remark 999. Synonym: mbp, mmbp, maltodextrin-binding protein, sine oculis homeobox homolog 1. Engineered: yes. Mutation: yes. Eyes absent homolog 2.

Source:

Escherichia coli, homo sapiens. Human. Organism_taxid: 562, 9606. Gene: b4034, jw3994, male, mbp, six1. Expressed in: escherichia coli. Expression_system_taxid: 562. Homo sapiens. Organism_taxid: 9606. Gene: eab1, eya2.

Resolution:

1.99Å

R-factor:

0.186

R-free:

0.224

Authors:

R.Zhao,A.N.Patrick

Key ref:

A.N.Patrick et al. (2013). Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome. Nat Struct Biol, 20, 447-453. PubMed id: 23435380 DOI: 10.1038/nsmb.2505

Date:

30-Mar-12

Release date:

27-Feb-13

PROCHECK

	Headers

	References

Protein chain

P0AEX9 (MALE_ECOLI) - Maltose/maltodextrin-binding periplasmic protein from Escherichia coli (strain K12)

Seq: Struc:

Seq: Struc:					396 a.a. 539 a.a.*

Protein chain

Q15475 (SIX1_HUMAN) - Homeobox protein SIX1 from Homo sapiens

Seq: Struc:

Seq: Struc:					284 a.a. 539 a.a.

Protein chain

O00167 (EYA2_HUMAN) - Eyes absent homolog 2 from Homo sapiens

Seq: Struc:

Seq: Struc:					538 a.a. 255 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 9 residue positions (black crosses)



		Enzyme reactions

Enzyme class 2:

Chain A: E.C.?

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Enzyme class 3:

Chain B: E.C.3.1.3.48 - protein-tyrosine-phosphatase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate

O-phospho-L-tyrosyl-[protein]	+	H2O	=	L-tyrosyl-[protein]	+	phosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1038/nsmb.2505	Nat Struct Biol 20:447-453 (2013)
PubMed id: 23435380

Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome.
A.N.Patrick, J.H.Cabrera, A.L.Smith, X.S.Chen, H.L.Ford, R.Zhao.

ABSTRACT

No abstract given.