PDBsum entry 4efb

Ligase/ligase inhibitor

PDB id: 4efb

Contents

Protein chain

313 a.a.

Ligands

SO4 ×2

NMN

0OW

Waters ×284

PDB id:

4efb

Name:

Ligase/ligase inhibitor

Title:

Crystal structure of DNA ligase

Structure:

DNA ligase. Chain: a. Synonym: polydeoxyribonucleotide synthase [nad(+)]. Engineered: yes

Source:

Synthetic: yes. Enterococcus faecalis. Organism_taxid: 1351. Other_details: this sequence occurs naturally in bacterial

Resolution:

2.20Å R-factor: 0.196 R-free: 0.277

Authors:

Y.Wei,T.Wang,P.Charifson,W.Xu

Key ref:

T.Wang et al. Crystal structure of DNA ligase. To be published, .

Date:

29-Mar-12 Release date: 03-Apr-13

Protein chain

Q837V6  (DNLJ_ENTFA) -  DNA ligase from Enterococcus faecalis (strain ATCC 700802 / V583)

Seq: 676 a.a.

Struc: 313 a.a.

Enzyme reactions

• Enzyme class: E.C.6.5.1.2 - Dna ligase (NAD(+)).
• Reaction: NAD⁺ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta- nicotinamide D-nucleotide

NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP (Bound ligand (Het Group name = NMN) matches with 73.08% similarity) + beta- nicotinamide D-nucleotide

Molecule diagrams generated from .mol files obtained from the KEGG ftp site