PDBsum entry 4eax

Hormone

PDB id: 4eax

Contents

Protein chains

108 a.a.

Ligands

S12 ×2

Waters ×60

PDB id:

4eax

Name:

Hormone

Title:

Mouse ngf in complex with lyso-ps

Structure:

Beta-nerve growth factor. Chain: a, b, c, d. Synonym: beta-ngf

Source:

Mus musculus. Mouse. Organism_taxid: 10090

Resolution:

2.30Å R-factor: 0.221 R-free: 0.259

Authors:

T.Jiang,Q.Tong

Key ref:

Q.Tong et al. (2012). Structural and functional insights into lipid-bound nerve growth factors. Faseb J, 26, 3811-3821. PubMed id: 22649032

Date:

23-Mar-12 Release date: 12-Sep-12

Protein chains

P01139  (NGF_MOUSE) -  Beta-nerve growth factor from Mus musculus

Seq: 241 a.a.

Struc: 108 a.a.

Faseb J 26:3811-3821 (2012)

PubMed id: 22649032

Structural and functional insights into lipid-bound nerve growth factors.

Q.Tong, F.Wang, H.Z.Zhou, H.L.Sun, H.Song, Y.Y.Shu, Y.Gong, W.T.Zhang, T.X.Cai, F.Q.Yang, J.Tang, T.Jiang.

ABSTRACT

Nerve growth factor (NGF) is a dimeric molecule that modulates the survival, proliferation, and differentiation of nervous cells and is also known to act on cells of the immune system and endocrine system. NGFs extracted from mouse submaxillary gland and cobra venom have different immunological behaviors, yet the underlying mechanism remains unclear. Here we report the crystal structure of the NGF purified from Chinese cobra Naja naja atra (cNGF), which unexpectedly reveals a 2-tailed lipid molecule that is embedded between the two protomers of the NGF homodimer. In addition, crystallographic analysis indicated that the purified mouse NGF(mNGF) is free from lipid but can bind lysophosphatidylserine (lyso-PS) in the same pocket as cNGF. Bioassays indicated that the binding of lipid molecules to cNGF and mNGF are essential for their mast cell activation activity and abates their p75(NTR) binding capacity. Taken together, these results suggest a new mechanism for the regulation of the function of NGF.-Tong, Q., Wang, F., Zhou, H.-Z., Sun, H.-L., Song, H., Shu, Y.-Y., Gong, Y., Zhang, W.-T., Cai, T.-X., Yang, F.-Q., Tang, J., Jiang, T. Structural and functional insights into lipid-bound nerve growth factors.