PDBsum entry 4e3c

Transferase

PDB id: 4e3c

Contents

Protein chain

(+ 0 more) 624 a.a.

PDB id:

4e3c

Name:

Transferase

Title:

X-ray crystal structure of human ikk2 in an active conformation

Structure:

Inhibitor of nuclear factor kappa-b kinase subunit beta. Chain: a, b, c, d, e, f. Fragment: unp residues 11-669. Synonym: i-kappa-b-kinase beta, ikk-b, ikk-beta, ikbkb, i-kappa-b kinase 2, ikk2, nuclear factor nf-kappa-b inhibitor kinase beta, nfkbikb. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ikbkb, ikkb. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108.

Resolution:

3.98Å R-factor: 0.267 R-free: 0.299

Authors:

S.Polley,D.B.Huang,A.V.Hauenstein,G.Ghosh,T.Huxford

Key ref:

S.Polley et al. X-Ray crystal structure of human ikk2 in an active conformation. Plos biol, .

Date:

09-Mar-12 Release date: 19-Jun-13

Protein chains

O14920  (IKKB_HUMAN) -  Inhibitor of nuclear factor kappa-B kinase subunit beta from Homo sapiens

Seq: 756 a.a.

Struc: 624 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺
• Enzyme class 3: E.C.2.7.11.10 - IkappaB kinase.
• Reaction: L-seryl-[I-kappa-B protein] + ATP = O-phospho-L-seryl-[I-kappa-B protein] + ADP + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site