PDBsum entry 4e2f

Transferase/protein binding

PDB id: 4e2f

Contents

Protein chains

(+ 0 more) 310 a.a.

(+ 0 more) 144 a.a.

Metals

_ZN ×6

Waters ×506

PDB id:

4e2f

Name:

Transferase/protein binding

Title:

Crystal structure of e. Coli aspartate transcarbamoylase k164e/e239k mutant in an intermediate state

Structure:

Aspartate carbamoyltransferase catalytic chain. Chain: i, k, g, c, a, e. Synonym: aspartate transcarbamylase, atcase. Engineered: yes. Mutation: yes. Aspartate carbamoyltransferase regulatory chain. Chain: d, b, j, l, h, f. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b4245, jw4204, pyrb. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: b4244, jw4203, pyri.

Resolution:

2.80Å R-factor: 0.217 R-free: 0.274

Authors:

W.Guo,E.R.Kantrowitz

Key ref:

W.Guo et al. (2012). Trapping and structure determination of an intermediate in the allosteric transition of aspartate transcarbamoylase. Proc Natl Acad Sci U S A, 109, 7741-7746. PubMed id: 22547808

Date:

08-Mar-12 Release date: 02-May-12

Protein chains

P0A786  (PYRB_ECOLI) -  Aspartate carbamoyltransferase catalytic subunit from Escherichia coli (strain K12)

Seq: 311 a.a.

Struc: 310 a.a.*

Protein chains

P0A7F3  (PYRI_ECOLI) -  Aspartate carbamoyltransferase regulatory chain from Escherichia coli (strain K12)

Seq: 153 a.a.

Struc: 144 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chains I, K, G, C, A, E: E.C.2.1.3.2 - aspartate carbamoyltransferase.
• Pathway: Pyrimidine Biosynthesis
• Reaction: carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H⁺
• Enzyme class 3: Chains D, B, J, L, H, F: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Proc Natl Acad Sci U S A 109:7741-7746 (2012)

PubMed id: 22547808

Trapping and structure determination of an intermediate in the allosteric transition of aspartate transcarbamoylase.

W.Guo, J.M.West, A.S.Dutton, H.Tsuruta, E.R.Kantrowitz.

ABSTRACT

No abstract given.