PDBsum entry 4d0c

Immune system

PDB id: 4d0c

Contents

Protein chains

276 a.a.

97 a.a.

Ligands

THR-ALA-GLY-GLN-SER-ASN-TYR-ASP-ARG-LEU

EDO

Waters ×31

PDB id:

4d0c

Name:

Immune system

Title:

Complex of a b21 chicken mhc class i molecule and a 10mer chicken peptide

Structure:

Mhc class i alpha chain 2. Chain: a. Fragment: extracellular domains, residues 1-291. Synonym: major histocompatibility complex class i glycoprotein haplotype b21. Engineered: yes. Beta-2-microglobulin. Chain: b. Fragment: residues 22-119.

Source:

Gallus gallus. Chicken. Organism_taxid: 9031. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: plyss rosetta cells. Other_details: b21 haplotype. Synthetic: yes. Other_details: synthetic peptide

Resolution:

2.81Å R-factor: 0.275 R-free: 0.282

Authors:

P.E.Chappell,P.Roversi,M.C.Harrison,L.E.Mears,J.F.Kaufman,S.M.Lea

Key ref:

P.Chappell et al. (2015). Expression levels of MHC class I molecules are inversely correlated with promiscuity of peptide binding. Elife, 4, e05345. PubMed id: 25860507 DOI: 10.7554/eLife.05345

Date:

25-Apr-14 Release date: 06-May-15

Supersedes:

2yf5

Protein chain

Q95601  (Q95601_CHICK) -  MHC class I alpha chain 2 from Gallus gallus

Seq: 355 a.a.

Struc: 276 a.a.*

Protein chain

P21611  (B2MG_CHICK) -  Beta-2-microglobulin from Gallus gallus

Seq: 119 a.a.

Struc: 97 a.a.

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

DOI no: 10.7554/eLife.05345

Elife 4:e05345 (2015)

PubMed id: 25860507

Expression levels of MHC class I molecules are inversely correlated with promiscuity of peptide binding.

P.Chappell, e.l. .K.Meziane, M.Harrison, �.�.Magiera, C.Hermann, L.Mears, A.G.Wrobel, C.Durant, L.L.Nielsen, S.Buus, N.Ternette, W.Mwangi, C.Butter, V.Nair, T.Ahyee, R.Duggleby, A.Madrigal, P.Roversi, S.M.Lea, J.Kaufman.

ABSTRACT

Highly polymorphic major histocompatibility complex (MHC) molecules are at the heart of adaptive immune responses, playing crucial roles in many kinds of disease and in vaccination. We report that breadth of peptide presentation and level of cell surface expression of class I molecules are inversely correlated in both chickens and humans. This relationship correlates with protective responses against infectious pathogens including Marek's disease virus leading to lethal tumours in chickens and human immunodeficiency virus infection progressing to AIDS in humans. We propose that differences in peptide binding repertoire define two groups of MHC class I molecules strategically evolved as generalists and specialists for different modes of pathogen resistance. We suggest that differences in cell surface expression level ensure the development of optimal peripheral T cell responses. The inverse relationship of peptide repertoire and expression is evidently a fundamental property of MHC molecules, with ramifications extending beyond immunology and medicine to evolutionary biology and conservation.