PDBsum entry 4cf5

Hydrolase

PDB id: 4cf5

Contents

Protein chains

223 a.a.

Waters ×242

PDB id:

4cf5

Name:

Hydrolase

Title:

Mutagenesis of a rhodobacteraceae l-haloacid dehalogenase

Structure:

L-haloacid dehalogenase. Chain: a, b, c, d. Engineered: yes. Mutation: yes

Source:

Rhodobacteraceae. Organism_taxid: 31989. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: codonplus-ripl.

Resolution:

2.34Å R-factor: 0.179 R-free: 0.250

Authors:

H.R.Novak,C.Sayer,M.N.Isupov,J.A.Littlechild

Key ref:

H.R.Novak et al. Mutagenesis of a rhodobacteraceae l-Haloacid dehaloge. To be published, .

Date:

13-Nov-13 Release date: 26-Nov-14

Protein chains

R4GRT2  (R4GRT2_9RHOB) -  (S)-2-haloacid dehalogenase from Paracoccaceae

Seq: 236 a.a.

Struc: 223 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.8.1.2 - (S)-2-haloacid dehalogenase.
• Pathway: 2-Haloacid Dehalogenase (configuration-inverting)
• Reaction: an (S)-2-haloacid + H2O = a (2R)-2-hydroxycarboxylate + a halide anion + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site