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PDBsum entry 4c8e
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PDB id:
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Lyase
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Title:
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Ispf (burkholderia cenocepacia) 2cmp complex
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Structure:
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2-c-methyl-d-erythritol 2,4-cyclodiphosphate synthase. Chain: a, b, c. Synonym: mecdp-synthase, mecpp-synthase, mecps, 2c-methyl-d- erythritol-2,4-cyclodiphosphate synthase. Engineered: yes
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Source:
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Burkholderia cenocepacia. Organism_taxid: 216591. Strain: j2315. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: gold. Other_details: genomic DNA
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Resolution:
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1.90Å
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R-factor:
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0.206
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R-free:
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0.251
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Authors:
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P.E.F.O'Rourke,J.Kalinowska-Tluscik,P.K.Fyfe,A.Dawson,W.N.Hunter
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Key ref:
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P.E.O'Rourke
et al.
(2014).
Crystal structures of IspF from Plasmodium falciparum and Burkholderia cenocepacia: comparisons inform antimicrobial drug target assessment.
Bmc Struct Biol,
14,
1.
PubMed id:
DOI:
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Date:
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30-Sep-13
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Release date:
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08-Jan-14
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PROCHECK
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Headers
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References
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B4EC22
(ISPF_BURCJ) -
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)
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Seq:
Struc:
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161 a.a.
161 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class:
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E.C.4.6.1.12
- 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase.
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Reaction:
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4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4- cyclic diphosphate + CMP
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4-CDP-2-C-methyl-D-erythritol 2-phosphate
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=
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2-C-methyl-D-erythritol 2,4- cyclic diphosphate
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+
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CMP
Bound ligand (Het Group name = )
corresponds exactly
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Cofactor:
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Mn(2+) or Mg(2+)
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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DOI no:
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Bmc Struct Biol
14:1
(2014)
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PubMed id:
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Crystal structures of IspF from Plasmodium falciparum and Burkholderia cenocepacia: comparisons inform antimicrobial drug target assessment.
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P.E.O'Rourke,
J.Kalinowska-Tłuścik,
P.K.Fyfe,
A.Dawson,
W.N.Hunter.
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ABSTRACT
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