PDBsum entry 4c3c

Plant protein

PDB id: 4c3c

Contents

Protein chain

207 a.a.

Ligands

TLA

Waters ×165

PDB id:

4c3c

Name:

Plant protein

Title:

Thaumatin refined against hatrx data for time-point 1

Structure:

Thaumatin-1. Chain: a. Synonym: thaumatin i

Source:

Thaumatococcus daniellii. Organism_taxid: 4621

Resolution:

1.80Å R-factor: 0.175 R-free: 0.200

Authors:

B.A.Yorke,G.S.Beddard,R.L.Owen,A.R.Pearson

Key ref:

B.A.Yorke et al. (2014). Time-resolved crystallography using the Hadamard transform. Nat Methods, 11, 1131-1134. PubMed id: 25282611 DOI: 10.1038/nmeth.3139

Date:

22-Aug-13 Release date: 03-Sep-14

Protein chain

P02883  (THM1_THADA) -  Thaumatin I from Thaumatococcus daniellii

Seq: 235 a.a.

Struc: 207 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1038/nmeth.3139

Nat Methods 11:1131-1134 (2014)

PubMed id: 25282611

Time-resolved crystallography using the Hadamard transform.

B.A.Yorke, G.S.Beddard, R.L.Owen, A.R.Pearson.

ABSTRACT

We describe a method for performing time-resolved X-ray crystallographic experiments based on the Hadamard transform, in which time resolution is defined by the underlying periodicity of the probe pulse sequence, and signal/noise is greatly improved over that for the fastest pump-probe experiments depending on a single pulse. This approach should be applicable on standard synchrotron beamlines and will enable high-resolution measurements of protein and small-molecule structural dynamics. It is also applicable to other time-resolved measurements where a probe can be encoded, such as pump-probe spectroscopy.