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PDBsum entry 4c2s
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PDB id:
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Transferase
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Title:
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Crystal structure of the fucosylgalactoside alpha n- acetylgalactosaminyltransferase (gta p156l mutant) in complex with udp and deoxy-h-antigen acceptor
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Structure:
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Histo-blood group abo system transferase. Chain: a, b. Fragment: extracellular catalytic domain, residues 64-354. Synonym: fucosylglycoprotein 3-alpha-galactosyltransferase, fucosylglycoprotein alpha-n-acetylgalactosaminyltransferase, glycoprotein-fucosylgalactoside alpha-n- acetylgalactosaminyltransferase, glycoprotein-fucosylgalactoside alpha-galactosyltransferase, histo-blood group a transferase, a transferase, histo-blood group b transferase, b transferase, nagat,
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 511693.
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Resolution:
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2.48Å
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R-factor:
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0.147
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R-free:
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0.182
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Authors:
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J.T.Weadge,M.Palcic,A.Henriksen
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Key ref:
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J.T.Wedge
et al.
Structural and biochemical characterization of the hu blood group a and b galactosyltransferases posessing pro156leu mutation.
To be published,
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Date:
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19-Aug-13
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Release date:
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11-Sep-13
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PROCHECK
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Headers
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References
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P16442
(BGAT_HUMAN) -
Histo-blood group ABO system transferase from Homo sapiens
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Seq:
Struc:
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354 a.a.
291 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 2 residue positions (black
crosses)
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Enzyme class 1:
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E.C.2.4.1.37
- fucosylgalactoside 3-alpha-galactosyltransferase.
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Reaction:
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an alpha-L-fucosyl-(1->2)-beta-D-galactosyl derivative + UDP-alpha-D- galactose = an alpha-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->2)]-beta-D- galactosyl derivative + UDP + H+
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alpha-L-fucosyl-(1->2)-beta-D-galactosyl derivative
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+
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UDP-alpha-D- galactose
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=
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alpha-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->2)]-beta-D- galactosyl derivative
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+
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UDP
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+
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H(+)
Bound ligand (Het Group name = )
corresponds exactly
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Enzyme class 2:
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E.C.2.4.1.40
- glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase.
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Reaction:
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an alpha-L-fucosyl-(1->2)-beta-D-galactosyl derivative + UDP-N-acetyl- alpha-D-galactosamine = an N-acetyl-alpha-D-galactosaminyl-(1->3)-[alpha- L-fucosyl-(1->2)]-beta-D-galactosyl derivative + UDP + H+
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alpha-L-fucosyl-(1->2)-beta-D-galactosyl derivative
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+
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UDP-N-acetyl- alpha-D-galactosamine
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=
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N-acetyl-alpha-D-galactosaminyl-(1->3)-[alpha- L-fucosyl-(1->2)]-beta-D-galactosyl derivative
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+
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UDP
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+
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H(+)
Bound ligand (Het Group name = )
corresponds exactly
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
