PDBsum entry 4bgd

Transcription

PDB id: 4bgd

Contents

Protein chains

1707 a.a.

248 a.a.

Ligands

ADP

PE5

Metals

_MG

Waters ×15

PDB id:

4bgd

Name:

Transcription

Title:

Crystal structure of brr2 in complex with the jab1/mpn domain of prp8

Structure:

Pre-mRNA-splicing helicase brr2. Chain: a. Fragment: residues 442-2163. Synonym: protein snu246. Engineered: yes. Pre-mRNA-splicing factor 8. Chain: c. Fragment: residues 2148-2395. Synonym: prp8, jab1/mpn.

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Strain: s288c. Expressed in: saccharomyces cerevisiae. Expression_system_taxid: 4932. Expression_system_taxid: 4932

Resolution:

3.10Å R-factor: 0.217 R-free: 0.270

Authors:

T.H.D.Nguyen,J.Li,K.Nagai

Key ref:

T.H.Nguyen et al. (2013). Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site. Structure, 21, 910-919. PubMed id: 23727230 DOI: 10.1016/j.str.2013.04.017

Date:

25-Mar-13 Release date: 29-May-13

Protein chain

P32639  (BRR2_YEAST) -  Pre-mRNA-splicing helicase BRR2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 2163 a.a.

Struc: 1707 a.a.

Protein chain

P33334  (PRP8_YEAST) -  Pre-mRNA-splicing factor 8 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 2413 a.a.

Struc: 248 a.a.

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.4.13 - Rna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

ATP + H2O = ADP (Bound ligand (Het Group name = ADP) corresponds exactly) + phosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.str.2013.04.017

Structure 21:910-919 (2013)

PubMed id: 23727230

Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site.

T.H.Nguyen, J.Li, W.P.Galej, H.Oshikane, A.J.Newman, K.Nagai.

ABSTRACT

The U5 small nuclear ribonucleoprotein particle (snRNP) helicase Brr2 disrupts the U4/U6 small nuclear RNA (snRNA) duplex and allows U6 snRNA to engage in an intricate RNA network at the active center of the spliceosome. Here, we present the structure of yeast Brr2 in complex with the Jab1/MPN domain of Prp8, which stimulates Brr2 activity. Contrary to previous reports, our crystal structure and mutagenesis data show that the Jab1/MPN domain binds exclusively to the N-terminal helicase cassette. The residues in the Jab1/MPN domain, whose mutations in human Prp8 cause the degenerative eye disease retinitis pigmentosa, are found at or near the interface with Brr2, clarifying its molecular pathology. In the cytoplasm, Prp8 forms a precursor complex with U5 snRNA, seven Smproteins, Snu114, and Aar2, but after nuclear import, Brr2 replaces Aar2 to form mature U5 snRNP. Our structure explains why Aar2 and Brr2 are mutually exclusive and provides important insights into the assembly of U5 snRNP.