PDBsum entry 4bai

Lyase

PDB id: 4bai

Contents

Protein chain

377 a.a.

Ligands

ACT ×2

Waters ×110

PDB id:

4bai

Name:

Lyase

Title:

Mycobacterium tuberculosis chorismate synthase before exposure to 266 nm uv laser

Structure:

Chorismate synthase. Chain: a. Synonym: 5-enolpyruvylshikimate-3-phosphate phospholyase. Engineered: yes

Source:

Mycobacterium tuberculosis. Organism_taxid: 1773. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.30Å R-factor: 0.183 R-free: 0.204

Authors:

P.J.B.Pereira,A.Royant,S.Panjikar,D.De Sanctis

Key ref:

P.J.Pereira et al. (2013). In-house UV radiation-damage-induced phasing of selenomethionine-labeled protein structures. J Struct Biol, 181, 89-94. PubMed id: 23178456

Date:

14-Sep-12 Release date: 17-Apr-13

Protein chain

P9WPY1  (AROC_MYCTU) -  Chorismate synthase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 401 a.a.

Struc: 377 a.a.

Enzyme reactions

• Enzyme class: E.C.4.2.3.5 - chorismate synthase.
• Pathway: Shikimate and Chorismate Biosynthesis
• Reaction: 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate
• Cofactor: FMN

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Struct Biol 181:89-94 (2013)

PubMed id: 23178456

In-house UV radiation-damage-induced phasing of selenomethionine-labeled protein structures.

P.J.Pereira, A.Royant, S.Panjikar, D.de Sanctis.

ABSTRACT

Selenomethionine labeling is the most common technique used in protein crystallography to derivatize recombinant proteins for experimental phasing using anomalous scattering at tunable synchrotron beamlines. Recently, it has been shown that UV radiation depletes electron density of selenium atoms of selenomethionine residues and that UV radiation-damage-induced phasing (equivalent to single isomorphous replacement) protocol can be applied to calculate experimental phases. Here we present the straightforward integration of a UV source with an in-house diffractometer. We show how this setup can extend the capabilities of a sealed tube X-ray generator and be used for experimental phasing of selenium-labeled proteins.