PDBsum entry 4ba6

Carbohydrate-binding protein

PDB id: 4ba6

Contents

Protein chain

128 a.a.

Ligands

GOL

Waters ×166

PDB id:

4ba6

Name:

Carbohydrate-binding protein

Title:

High resolution structure of thE C-terminal family 65 carbohydrate binding module (cbm65b) of endoglucanase cel5a from eubacterium cellulosolvens

Structure:

Endoglucanase cel5a. Chain: a. Fragment: c-terminal family 65 carbohydrate binding module (cbm65b), residues 581-713. Engineered: yes. Other_details: methionine 620 (met a620) has been oxidised to methionine sulphoxide (sme 620)

Source:

Eubacterium cellulosolvens. Organism_taxid: 29322. Gene: cel5a. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: tuner

Resolution:

1.42Å R-factor: 0.171 R-free: 0.197

Authors:

I.Venditto,A.S.Luis,A.Basle,M.Temple,L.M.A.Ferreira,C.M.G.A.Fontes, H.J.Gilbert,S.Najmudin

Key ref:

A.S.Luís et al. (2013). Understanding how noncatalytic carbohydrate binding modules can display specificity for xyloglucan. J Biol Chem, 288, 4799-4809. PubMed id: 23229556 DOI: 10.1074/jbc.M112.432781

Date:

11-Sep-12 Release date: 19-Dec-12

Protein chain

Q3LHN3  (Q3LHN3_EUBCE) -  Endoglucanase cel5A from Eubacterium cellulosolvens

Seq: 1148 a.a.

Struc: 128 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1074/jbc.M112.432781

J Biol Chem 288:4799-4809 (2013)

PubMed id: 23229556

Understanding how noncatalytic carbohydrate binding modules can display specificity for xyloglucan.

A.S.Luís, I.Venditto, M.J.Temple, A.Rogowski, A.Baslé, J.Xue, J.P.Knox, J.A.Prates, L.M.Ferreira, C.M.Fontes, S.Najmudin, H.J.Gilbert.

ABSTRACT

No abstract given.