PDBsum entry 4b1h

Hydrolase

PDB id: 4b1h

Contents

Protein chain

507 a.a.

Ligands

BME

DTV

GOL ×2

AR6 ×2

SO4 ×2

Waters ×378

PDB id:

4b1h

Name:

Hydrolase

Title:

Structure of human parg catalytic domain in complex with adp-ribose

Structure:

Poly(adp-ribose) glycohydrolase. Chain: a. Fragment: catalytic domain, residues 448-976. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: gold.

Resolution:

2.00Å R-factor: 0.205 R-free: 0.241

Authors:

C.Brassington,J.Ellston,G.Hassall,G.Holdgate,M.Mcalister,G.Smith, J.A.Tucker,M.Watson

Key ref:

J.A.Tucker et al. (2012). Structures of the human poly (ADP-ribose) glycohydrolase catalytic domain confirm catalytic mechanism and explain inhibition by ADP-HPD derivatives. Plos One, 7, e50889. PubMed id: 23251397

Date:

10-Jul-12 Release date: 19-Dec-12

Protein chain

Q86W56  (PARG_HUMAN) -  Poly(ADP-ribose) glycohydrolase from Homo sapiens

Seq: 976 a.a.

Struc: 507 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.143 - poly(ADP-ribose) glycohydrolase.
• Pathway: Poly(ADP-ribose) Glycohydrolase
• Reaction: [(1''->2')-ADP-alpha-D-ribose](n) + H2O = [(1''->2')-ADP-alpha-D- ribose](n-1) + ADP-D-ribose

[(1''->2')-ADP-alpha-D-ribose](n) + H2O = [(1''->2')-ADP-alpha-D- ribose](n-1) + ADP-D-ribose (Bound ligand (Het Group name = AR6) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

Plos One 7:e50889 (2012)

PubMed id: 23251397

Structures of the human poly (ADP-ribose) glycohydrolase catalytic domain confirm catalytic mechanism and explain inhibition by ADP-HPD derivatives.

J.A.Tucker, N.Bennett, C.Brassington, S.T.Durant, G.Hassall, G.Holdgate, M.McAlister, J.W.Nissink, C.Truman, M.Watson.

ABSTRACT

No abstract given.