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PDBsum entry 4b0c
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PDB id:
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Lyase
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Title:
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Crystal structure of 3-hydroxydecanoyl-acyl carrier protein dehydratase (faba) from pseudomonas aeruginosa in complex with 3- (pentylthio)-4h-1,2,4-triazole
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Structure:
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3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase. Chain: a, b, c, d, e. Synonym: beta-hydroxydecanoyl thioester dehydrase. Engineered: yes
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Source:
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Pseudomonas aeruginosa. Organism_taxid: 287. Strain: pa01. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: tuner.
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Resolution:
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2.70Å
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R-factor:
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0.206
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R-free:
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0.232
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Authors:
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L.Moynie,S.A.Mcmahon,F.G.Duthie,J.H.Naismith
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Key ref:
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L.Moynié
et al.
(2013).
Structural insights into the mechanism and inhibition of the β-hydroxydecanoyl-acyl carrier protein dehydratase from Pseudomonas aeruginosa.
J Mol Biol,
425,
365-377.
PubMed id:
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Date:
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01-Jul-12
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Release date:
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28-Nov-12
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PROCHECK
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Headers
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References
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O33877
(FABA_PSEAE) -
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
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Seq:
Struc:
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171 a.a.
168 a.a.*
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Key: |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class 2:
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E.C.4.2.1.59
- 3-hydroxyacyl-[acyl-carrier-protein] dehydratase.
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Reaction:
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a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O
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(3R)-3-hydroxyacyl-[acyl-carrier protein]
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=
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trans-2-enoyl-[acyl- carrier protein]
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+
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H(2)O
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Enzyme class 3:
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E.C.5.3.3.14
- trans-2-decenoyl-[acyl-carrier-protein] isomerase.
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Reaction:
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(2E)-decenoyl-[ACP] = (3Z)-decenoyl-[ACP]
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(2E)-decenoyl-[ACP]
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=
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(3Z)-decenoyl-[ACP]
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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J Mol Biol
425:365-377
(2013)
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PubMed id:
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Structural insights into the mechanism and inhibition of the β-hydroxydecanoyl-acyl carrier protein dehydratase from Pseudomonas aeruginosa.
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L.Moynié,
S.M.Leckie,
S.A.McMahon,
F.G.Duthie,
A.Koehnke,
J.W.Taylor,
M.S.Alphey,
R.Brenk,
A.D.Smith,
J.H.Naismith.
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ABSTRACT
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Links
