PDBsum entry 4awt

Oxidoreductase

PDB id: 4awt

Contents

Protein chain

364 a.a.

Ligands

FMN

SO4

BOG

01F

PE4

Waters ×621

PDB id:

4awt

Name:

Oxidoreductase

Title:

Crystal structure of the reduced shewanella yellow enzyme 1 (sye1) m25l mutant

Structure:

Sye1. Chain: a. Synonym: oxidoreductase, fmn-binding. Engineered: yes. Mutation: yes. Other_details: n-terminal t2 is involved in an n, o five ring acetal by reaction with ho-ch2-ch2-o-ch2-cho, a peg400 breakdown product.

Source:

Shewanella oneidensis. Organism_taxid: 211586. Strain: mr-1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

0.98Å R-factor: 0.095 R-free: 0.106

Authors:

J.Elegheert,A.Brige,S.N.Savvides

Key ref:

J.Elegheert et al. Modulation of isoalloxazine ring planarity influences electronic properties in old yellow enzymes. To be published, .

Date:

05-Jun-12 Release date: 26-Jun-13

Protein chain

Q8EEC8  (Q8EEC8_SHEON) -  NADH:flavin oxidoreductase Sye1 from Shewanella oneidensis (strain ATCC 700550 / JCM 31522 / CIP 106686 / LMG 19005 / NCIMB 14063 / MR-1)

Seq: 365 a.a.

Struc: 364 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.6.99.1 - Nadph dehydrogenase.
• Reaction: A + NADPH + H⁺ = AH2 + NADP⁺
• Cofactor: FMN or FAD

FMN (Bound ligand (Het Group name = FMN) corresponds exactly) or FAD

Molecule diagrams generated from .mol files obtained from the KEGG ftp site