PDBsum entry 4as2

Hydrolase

PDB id: 4as2

Contents

Protein chains

327 a.a.

Ligands

BTB ×8

Metals

IOD ×13

_MG ×4

_CL ×4

Waters ×814

PDB id:

4as2

Name:

Hydrolase

Title:

Pseudomonas aeruginosa phosphorylcholine phosphatase. Monoclinic form

Structure:

Phosphorylcholine phosphatase. Chain: a, b, c, d. Fragment: residues 23-349. Engineered: yes

Source:

Pseudomonas aeruginosa. Organism_taxid: 287. Expressed in: escherichia coli. Expression_system_taxid: 511693. Expression_system_variant: codonplus.

Resolution:

2.12Å R-factor: 0.157 R-free: 0.210

Authors:

L.Infantes,L.H.Otero,A.Albert

Key ref:

L.Infantes et al. (2012). The structural domains of Pseudomonas aeruginosa phosphorylcholine phosphatase cooperate in substrate hydrolysis: 3D structure and enzymatic mechanism. J Mol Biol, 423, 503-514. PubMed id: 22922065

Date:

27-Apr-12 Release date: 22-Aug-12

Protein chains

Q9HTR2  (PCHP_PSEAE) -  Phosphorylcholine phosphatase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 349 a.a.

Struc: 327 a.a.

Enzyme reactions

• Enzyme class: E.C.3.1.3.75 - phosphoethanolamine/phosphocholine phosphatase.
• Reaction:
  1. phosphoethanolamine + H2O = ethanolamine + phosphate
  2. phosphocholine + H2O = choline + phosphate

phosphocholine + H2O = choline (Bound ligand (Het Group name = BTB) matches with 40.00% similarity) + phosphate

• Cofactor: Mg(2+) or cobalt cation or Mn(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Mol Biol 423:503-514 (2012)

PubMed id: 22922065

The structural domains of Pseudomonas aeruginosa phosphorylcholine phosphatase cooperate in substrate hydrolysis: 3D structure and enzymatic mechanism.

L.Infantes, L.H.Otero, P.R.Beassoni, C.Boetsch, A.T.Lisa, C.E.Domenech, A.Albert.

ABSTRACT

No abstract given.