PDBsum entry 4ar5

Electron transport

PDB id: 4ar5

Contents

Protein chain

54 a.a.

Ligands

DOD ×135

Metals

_FE

PDB id:

4ar5

Name:

Electron transport

Title:

X-ray crystallographic structure of the oxidised form perdeuterated pyrococcus furiosus rubredoxin in d2o at 295k (in quartz capillary) to 1.00 angstrom resolution.

Structure:

Rubredoxin. Chain: a. Synonym: rd. Engineered: yes. Other_details: oxidised state (fe iii)

Source:

Pyrococcus furiosus. Organism_taxid: 2261. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.00Å R-factor: 0.110 R-free: 0.119

Authors:

M.G.Cuypers,S.A.Mason,M.P.Blakeley,E.P.Mitchell,M.Haertlein, V.T.Forsyth

Key ref:

M.G.Cuypers et al. (2013). Near-atomic resolution neutron crystallography on perdeuterated Pyrococcus furiosus rubredoxin: implication of hydronium ions and protonation state equilibria in redox changes. Angew Chem Int Ed Engl, 52, 1022-1025. PubMed id: 23225503

Date:

20-Apr-12 Release date: 19-Dec-12

Protein chain

P24297  (RUBR_PYRFU) -  Rubredoxin from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Seq: 54 a.a.

Struc: 54 a.a.

Angew Chem Int Ed Engl 52:1022-1025 (2013)

PubMed id: 23225503

Near-atomic resolution neutron crystallography on perdeuterated Pyrococcus furiosus rubredoxin: implication of hydronium ions and protonation state equilibria in redox changes.

M.G.Cuypers, S.A.Mason, M.P.Blakeley, E.P.Mitchell, M.Haertlein, V.T.Forsyth.

ABSTRACT

Neutron crystallographic analyses at near-atomic resolution are presented for both reduced and oxidized forms of perdeuterated Pyrococcus furiosus rubredoxin, a small iron-sulfur redox protein with remarkable thermostability. Hydronium ions may play a key role in the protonation and charge-transfer processes associated with the oxidized and reduced forms of the protein. Picture: overall structure showing D(3) O(+) ions (red and gray molecules).