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PDBsum entry 4a5o
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Oxidoreductase
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PDB id
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4a5o
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PDB id:
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| Name: |
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Oxidoreductase
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Title:
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Crystal structure of pseudomonas aeruginosa n5, n10- methylenetetrahydrofolate dehydrogenase-cyclohydrolase (fold)
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Structure:
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Bifunctional protein fold. Chain: a, b, c, d. Synonym: n5\,n10-methylenetetrahydrofolate dehydrogenase- cyclohydrolase, methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase. Engineered: yes
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Source:
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Pseudomonas aeruginosa pao1. Organism_taxid: 208964. Expressed in: escherichia coli. Expression_system_taxid: 469008.
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Resolution:
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2.20Å
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R-factor:
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0.236
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R-free:
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0.279
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Authors:
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T.C.Eadsforth,M.Gardiner,F.V.Maluf,S.Mcelroy,D.James,J.Frearson, D.Gray,W.N.Hunter
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Key ref:
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T.C.Eadsforth
et al.
(2012).
Assessment of Pseudomonas aeruginosa N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase as a potential antibacterial drug target.
Plos One,
7,
e35973.
PubMed id:
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Date:
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26-Oct-11
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Release date:
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16-Nov-11
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PROCHECK
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Headers
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References
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Q9I2U6
(FOLD_PSEAE) -
Bifunctional protein FolD from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
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Seq:
Struc:
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284 a.a.
283 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Enzyme class 1:
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E.C.1.5.1.5
- methylenetetrahydrofolate dehydrogenase (NADP(+)).
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Pathway:
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Folate Coenzymes
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Reaction:
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(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP+ = (6R)-5,10- methenyltetrahydrofolate + NADPH
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(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate
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+
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NADP(+)
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=
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(6R)-5,10- methenyltetrahydrofolate
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+
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NADPH
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Enzyme class 2:
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E.C.3.5.4.9
- methenyltetrahydrofolate cyclohydrolase.
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Pathway:
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Reaction:
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(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H+
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5,10-methenyltetrahydrofolate
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+
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H2O
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=
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(6S)-10-formyltetrahydrofolate
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+
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H(+)
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Plos One
7:e35973
(2012)
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PubMed id:
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Assessment of Pseudomonas aeruginosa N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase as a potential antibacterial drug target.
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T.C.Eadsforth,
M.Gardiner,
F.V.Maluf,
S.McElroy,
D.James,
J.Frearson,
D.Gray,
W.N.Hunter.
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ABSTRACT
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