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PDBsum entry 4wn5
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Transcription
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PDB id
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4wn5
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PDB id:
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| Name: |
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Transcription
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Title:
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Crystal structure of thE C-terminal per-arnt-sim (pasb) of human hif- 3alpha9 bound to 18:1-1-monoacylglycerol
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Structure:
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Hypoxia-inducible factor 3-alpha. Chain: a, b. Fragment: unp residues 235-345. Synonym: hif3-alpha,basic-helix-loop-helix-pas protein mop7,class e basic helix-loop-helix protein 17,bhlhe17,hif3-alpha-1,inhibitory pas domain protein,ipas,member of pas protein 7,pas domain-containing protein 7. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: hif3a, bhlhe17, mop7, pasd7. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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1.15Å
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R-factor:
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0.123
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R-free:
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0.141
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Authors:
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A.M.Fala,J.F.Oliveira,S.M.Dias,A.L.Ambrosio
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Key ref:
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A.M.Fala
et al.
(2015).
Unsaturated fatty acids as high-affinity ligands of the C-terminal Per-ARNT-Sim domain from the Hypoxia-inducible factor 3α.
Sci Rep,
5,
12698.
PubMed id:
DOI:
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Date:
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10-Oct-14
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Release date:
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05-Aug-15
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PROCHECK
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Headers
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References
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Q9Y2N7
(HIF3A_HUMAN) -
Hypoxia-inducible factor 3-alpha from Homo sapiens
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Seq:
Struc:
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669 a.a.
108 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 2 residue positions (black
crosses)
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DOI no:
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Sci Rep
5:12698
(2015)
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PubMed id:
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Unsaturated fatty acids as high-affinity ligands of the C-terminal Per-ARNT-Sim domain from the Hypoxia-inducible factor 3α.
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A.M.Fala,
J.F.Oliveira,
D.Adamoski,
J.A.Aricetti,
M.M.Dias,
M.V.B.Dias,
M.L.Sforça,
P.S.Lopes-de-Oliveira,
S.A.Rocco,
C.Caldana,
S.M.G.Dias,
A.L.B.Ambrosio.
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ABSTRACT
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Hypoxia-inducible transcription factors (HIF) form heterodimeric complexes that
mediate cell responses to hypoxia. The oxygen-dependent stability and activity
of the HIF-α subunits is traditionally associated to post-translational
modifications such as hydroxylation, acetylation, ubiquitination, and
phosphorylation. Here we report novel evidence showing that unsaturated fatty
acids are naturally occurring, non-covalent structural ligands of HIF-3α, thus
providing the initial framework for exploring its exceptional role as a lipid
sensor under hypoxia.
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