PDBsum entry 4us6

Isomerase

PDB id: 4us6

Contents

Protein chains

386 a.a.

Ligands

GOL ×6

Metals

_MG ×3

_NA ×2

_CA ×2

Waters ×938

PDB id:

4us6

Name:

Isomerase

Title:

New crystal form of glucose isomerase grown in short peptide supramolecular hydrogels

Structure:

Xylose isomerase. Chain: a, b. Ec: 5.3.1.5

Source:

Streptomyces rubiginosus. Organism_taxid: 1929

Resolution:

1.20Å R-factor: 0.108 R-free: 0.132

Authors:

J.A.Gavira,M.Conejero-Muriel,J.J.Diaz-Mochon,L.Alvarez De Cienfuegos

Key ref:

M.Conejero-Muriel et al. (2015). Influence of the chirality of short peptide supramolecular hydrogels in protein crystallogenesis. Chem Commun (camb), 51, 3862-3865. PubMed id: 25655841 DOI: 10.1039/c4cc09024a

Date:

03-Jul-14 Release date: 13-May-15

Protein chains

P24300  (XYLA_STRRU) -  Xylose isomerase from Streptomyces rubiginosus

Seq: 388 a.a.

Struc: 386 a.a.

Enzyme reactions

• Enzyme class: E.C.5.3.1.5 - xylose isomerase.
• Reaction: alpha-D-xylose = alpha-D-xylulofuranose

alpha-D-xylose (Bound ligand (Het Group name = GOL) matches with 60.00% similarity) = alpha-D-xylulofuranose

• Cofactor: Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1039/c4cc09024a

Chem Commun (camb) 51:3862-3865 (2015)

PubMed id: 25655841

Influence of the chirality of short peptide supramolecular hydrogels in protein crystallogenesis.

M.Conejero-Muriel, J.A.Gavira, E.Pineda-Molina, A.Belsom, M.Bradley, M.Moral, J.d.e. .D.García-López Durán, A.Luque González, J.J.Díaz-Mochón, R.Contreras-Montoya, �.�.Martínez-Peragón, J.M.Cuerva, L.Álvarez de Cienfuegos.

ABSTRACT

For the first time the influence of the chirality of the gel fibers in protein crystallogenesis has been studied. Enantiomeric hydrogels 1 and 2 were tested with model proteins lysozyme and glucose isomerase and a formamidase extracted from B. cereus. Crystallization behaviour and crystal quality of these proteins in both hydrogels are presented and compared.