PDBsum entry 4roc

Transcription

PDB id: 4roc

Contents

Protein chains

302 a.a.

178 a.a.

DNA/RNA

Metals

_MG

Waters ×379

PDB id:

4roc

Name:

Transcription

Title:

Human tfiib-related factor 2 (brf2) and tbp bound to u6#2 promoter

Structure:

Transcription factor iiib 50 kda subunit. Chain: a. Synonym: tfiiib50, htfiiib50, b-related factor 2, brf-2, hbrfu. Engineered: yes. Tata-box-binding protein. Chain: b. Synonym: tata sequence-binding protein, tata-binding factor, tata-box factor, transcription initiation factor tfiid tbp subunit. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: brf2, brfu, pro1470. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: tbp, gtf2d1, tf2d, tfiid. Synthetic: yes. Synthetic construct.

Resolution:

1.90Å R-factor: 0.187 R-free: 0.215

Authors:

A.Vannini,J.Gouge,K.Satia,N.Guthertz

Key ref:

J.Gouge et al. (2015). Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2. Cell, 163, 1375-1387. PubMed id: 26638071 DOI: 10.1016/j.cell.2015.11.005

Date:

28-Oct-14 Release date: 30-Dec-15

Protein chain

Q9HAW0  (BRF2_HUMAN) -  Transcription factor IIIB 50 kDa subunit from Homo sapiens

Seq: 419 a.a.

Struc: 302 a.a.

Protein chain

P20226  (TBP_HUMAN) -  TATA-box-binding protein from Homo sapiens

Seq: 339 a.a.

Struc: 178 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

T-T-G-A-A-G-G-G-C-T-T-A-A-A-A-T-A-G-G-T-G-T-G-A-C-A-G 27 bases

C-T-G-T-C-A-C-A-C-C-T-A-T-T-T-T-A-A-G-C-C-C-T-T-C-A-A 27 bases

Enzyme reactions

• Enzyme class: Chains A, B: E.C.?

DOI no: 10.1016/j.cell.2015.11.005

Cell 163:1375-1387 (2015)

PubMed id: 26638071

Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2.

J.Gouge, K.Satia, N.Guthertz, M.Widya, A.J.Thompson, P.Cousin, O.Dergai, N.Hernandez, A.Vannini.

ABSTRACT

TFIIB-related factor 2 (Brf2) is a member of the family of TFIIB-like core transcription factors. Brf2 recruits RNA polymerase (Pol) III to type III gene-external promoters, including the U6 spliceosomal RNA and selenocysteine tRNA genes. Found only in vertebrates, Brf2 has been linked to tumorigenesis but the underlying mechanisms remain elusive. We have solved crystal structures of a human Brf2-TBP complex bound to natural promoters, obtaining a detailed view of the molecular interactions occurring at Brf2-dependent Pol III promoters and highlighting the general structural and functional conservation of human Pol II and Pol III pre-initiation complexes. Surprisingly, our structural and functional studies unravel a Brf2 redox-sensing module capable of specifically regulating Pol III transcriptional output in living cells. Furthermore, we establish Brf2 as a central redox-sensing transcription factor involved in the oxidative stress pathway and provide a mechanistic model for Brf2 genetic activation in lung and breast cancer.