PDBsum entry 4re2

Hydrolase

PDB id: 4re2

Contents

Protein chain

482 a.a.

Ligands

MVL

TRS

EPE

GOL ×2

Waters ×323

PDB id:

4re2

Name:

Hydrolase

Title:

Different transition state conformations for the hydrolysis of beta- mannosides and beta-glucosides in the rice os7bglu26 family gh1 beta- mannosidase/beta-glucosidase

Structure:

Beta-mannosidase/beta-glucosidase. Chain: a. Synonym: os7bglu26. Engineered: yes

Source:

Oryza sativa indica group. Indian rice. Organism_taxid: 39946. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.00Å R-factor: 0.190 R-free: 0.225

Authors:

A.Tankrathok,J.Iglesias-Fernandez,R.J.Williams,Z.Hakki,R.C.Robinson, M.Hrmova,C.Rovira,S.J.Williams,J.R.Ketudat Cairns

Key ref:

A.Tankrathok et al. A single glycosidase harnesses different pyranoside r transition state conformations for hydrolysis of mann and glucosides. Acs catalysis, .

Date:

21-Sep-14 Release date: 16-Sep-15

Protein chain

A2YPH1  (A2YPH1_ORYSI) -  Beta-glucosidase from Oryza sativa subsp. indica

Seq: 510 a.a.

Struc: 482 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.25 - beta-mannosidase.
• Reaction: Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.