PDBsum entry 4qk2

Lyase

PDB id: 4qk2

Contents

Protein chain

258 a.a.

Ligands

GOL

Metals

_ZN

Waters ×275

PDB id:

4qk2

Name:

Lyase

Title:

Structural and catalytic effects of proline substitution and surface loop deletion in the extended active site of human carbonic anhydrase ii - e234p

Structure:

Carbonic anhydrase 2. Chain: a. Synonym: carbonate dehydratase ii, carbonic anhydrasE C, cac, carbonic anhydrase ii, ca-ii. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ca2. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.52Å R-factor: 0.160 R-free: 0.178

Authors:

C.D.Boone,V.Rasi,R.Mckenna

Key ref:

C.D.Boone et al. (2015). Structural and catalytic effects of proline substitution and surface loop deletion in the extended active site of human carbonic anhydrase II. Febs J, 282, 1445-1457. PubMed id: 25683338 DOI: 10.1111/febs.13232

Date:

05-Jun-14 Release date: 04-Mar-15

Protein chain

P00918  (CAH2_HUMAN) -  Carbonic anhydrase 2 from Homo sapiens

Seq: 260 a.a.

Struc: 258 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.2.1.1 - carbonic anhydrase.
• Reaction: hydrogencarbonate + H⁺ = CO2 + H2O
• Cofactor: Zn(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1111/febs.13232

Febs J 282:1445-1457 (2015)

PubMed id: 25683338

Structural and catalytic effects of proline substitution and surface loop deletion in the extended active site of human carbonic anhydrase II.

C.D.Boone, V.Rasi, C.Tu, R.McKenna.

ABSTRACT

No abstract given.