PDBsum entry 4qa8

Lipid transport

PDB id: 4qa8

Contents

Protein chain

210 a.a.

Ligands

PJZ

Waters ×156

PDB id:

4qa8

Name:

Lipid transport

Title:

Crystal structure of lprf from mycobacterium bovis

Structure:

Putative lipoprotein lprf. Chain: a. Fragment: unp residues 40-261. Engineered: yes

Source:

Mycobacterium bovis. Organism_taxid: 233413. Strain: atcc baa-935 / af2122/97. Gene: lprf, mb1403. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.10Å R-factor: 0.165 R-free: 0.189

Authors:

N.C.Ha,L.Jiao,J.S.Kim

Key ref:

J.S.Kim et al. (2014). Crystal structure and functional implications of LprF from Mycobacterium tuberculosis and M. bovis. Acta Crystallogr D Biol Crystallogr, 70, 2619-2630. PubMed id: 25286846 DOI: 10.1107/S1399004714016599

Date:

02-May-14 Release date: 22-Oct-14

Protein chain

P65315  (LPRF_MYCBO) -  Putative diacylated glycolipid transporter LprF from Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)

Seq: 261 a.a.

Struc: 210 a.a.

DOI no: 10.1107/S1399004714016599

Acta Crystallogr D Biol Crystallogr 70:2619-2630 (2014)

PubMed id: 25286846

Crystal structure and functional implications of LprF from Mycobacterium tuberculosis and M. bovis.

J.S.Kim, L.Jiao, J.I.Oh, N.C.Ha, Y.H.Kim.

ABSTRACT

The Gram-positive bacteria Mycobacterium tuberculosis and M. bovis are causative agents of tuberculosis in humans and cattle. The lipoprotein LprF is found in M. tuberculosis and M. bovis but not in the nonpathogenic M. smegmatis. To date, the role of LprF remains to be elucidated. In this study, the crystal structure of LprF has been determined at 1.1 Å resolution. The overall structure is similar to that of a homologue, LprG, with a central hydrophobic cavity that binds a triacylated glycolipid. LprF exhibited a central cavity structure similar to that of LprG, but with a smaller cavity that binds two alkyl chains. Consistently, subsequent mass-spectrometric analysis revealed that the bound ligand was a diacylated glycolipid, as found in the structure. Furthermore, an increased ratio of lipoarabinomannan to lipomannan in the mycobacterial cell wall was observed when lprF was introduced into M. smegmatis. These observations suggested that LprF transfers the diacylated glycolipid from the plasma membrane to the cell wall, which might be related to the pathogenesis of the bacteria.