PDBsum entry 4pir

Transport protein

PDB id: 4pir

Contents

Protein chains

383 a.a.

122 a.a.

Ligands

NAG-NAG ×9

NAG-NAG-BMA ×5

SO4 ×3

NAG ×2

Metals

_CL ×5

PDB id:

4pir

Name:

Transport protein

Title:

X-ray structure of the mouse serotonin 5-ht3 receptor

Structure:

5-hydroxytryptamine receptor 3a. Chain: a, b, c, d, e. Synonym: 5-ht3a,5-hydroxytryptamine receptor 3,5-ht3r,serotonin receptor 3a,serotonin-gated ion channel receptor. Engineered: yes. Vhh15. Chain: f, g, h, i, j. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: htr3a, 5ht3, htr3. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293. Lama glama. Organism_taxid: 9844.

Resolution:

3.50Å R-factor: 0.219 R-free: 0.257

Authors:

G.Hassaine,C.Deluz,L.Grasso,R.Wyss,M.B.Tol,R.Hovius,A.Graff, H.Stahlberg,T.Tomizaki,A.Desmyter,C.Moreau,X.-D.Li,F.Poitevin, H.Vogel,H.Nury

Key ref:

G.Hassaine et al. (2014). X-ray structure of the mouse serotonin 5-HT3 receptor. Nature, 512, 276-281. PubMed id: 25119048 DOI: 10.1038/nature13552

Date:

09-May-14 Release date: 06-Aug-14

Protein chains

P23979  (5HT3A_MOUSE) -  5-hydroxytryptamine receptor 3A from Mus musculus

Seq: 489 a.a.

Struc: 383 a.a.

Protein chains

No UniProt id for this chain

Struc: 122 a.a.

DOI no: 10.1038/nature13552

Nature 512:276-281 (2014)

PubMed id: 25119048

X-ray structure of the mouse serotonin 5-HT3 receptor.

G.Hassaine, C.Deluz, L.Grasso, R.Wyss, M.B.Tol, R.Hovius, A.Graff, H.Stahlberg, T.Tomizaki, A.Desmyter, C.Moreau, X.D.Li, F.Poitevin, H.Vogel, H.Nury.

ABSTRACT

Neurotransmitter-gated ion channels of the Cys-loop receptor family mediate fast neurotransmission throughout the nervous system. The molecular processes of neurotransmitter binding, subsequent opening of the ion channel and ion permeation remain poorly understood. Here we present the X-ray structure of a mammalian Cys-loop receptor, the mouse serotonin 5-HT3 receptor, at 3.5 Å resolution. The structure of the proteolysed receptor, made up of two fragments and comprising part of the intracellular domain, was determined in complex with stabilizing nanobodies. The extracellular domain reveals the detailed anatomy of the neurotransmitter binding site capped by a nanobody. The membrane domain delimits an aqueous pore with a 4.6 Å constriction. In the intracellular domain, a bundle of five intracellular helices creates a closed vestibule where lateral portals are obstructed by loops. This 5-HT3 receptor structure, revealing part of the intracellular domain, expands the structural basis for understanding the operating mechanism of mammalian Cys-loop receptors.