PDBsum entry 4p4e

Hydrolase

PDB id: 4p4e

Contents

Protein chain

694 a.a.

Ligands

NAG-NAG

NAG-NAG-FUC ×2

NAG-NAG-BMA

NAG-NAG-BMA-MAN

NAG ×2

2G4

Metals

_ZN ×2

_CA

_CL

Waters ×562

PDB id:

4p4e

Name:

Hydrolase

Title:

X-ray structure of human glutamate carboxypeptidase ii (gcpii) in complex with a phosphoramidate inhibitor mp1d

Structure:

Glutamate carboxypeptidase 2. Chain: a. Fragment: unp residues 44-750. Synonym: cell growth-inhibiting gene 27 protein,folate hydrolase 1, folylpoly-gamma-glutamate carboxypeptidase,fgcp,glutamate carboxypeptidase ii,gcpii,membrane glutamate carboxypeptidase,mgcp,n- acetylated-alpha-linked acidic dipeptidase i,naaladase i,prostate- specific membrane antigen,psma,pteroylpoly-gamma-glutamate carboxypeptidase.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: folh1, folh, naalad1, psm, psma, gig27. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227. Expression_system_cell_line: schneider's s2 cells.

Resolution:

1.67Å R-factor: 0.154 R-free: 0.183

Authors:

C.Barinka

Key ref:

Z.Novakova et al. (2016). Design of composite inhibitors targeting glutamate carboxypeptidase II: the importance of effector functionalities. Febs J, 283, 130-143. PubMed id: 26460595 DOI: 10.1111/febs.13557

Date:

12-Mar-14 Release date: 20-May-15

Protein chain

Q04609  (FOLH1_HUMAN) -  Glutamate carboxypeptidase 2 from Homo sapiens

Seq: 750 a.a.

Struc: 694 a.a.

Enzyme reactions

• Enzyme class: E.C.3.4.17.21 - glutamate carboxypeptidase Ii.
• Reaction: Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.
• Cofactor: Zn(2+)

DOI no: 10.1111/febs.13557

Febs J 283:130-143 (2016)

PubMed id: 26460595

Design of composite inhibitors targeting glutamate carboxypeptidase II: the importance of effector functionalities.

Z.Novakova, J.Cerny, C.J.Choy, J.R.Nedrow, J.K.Choi, J.Lubkowski, C.E.Berkman, C.Barinka.

ABSTRACT

No abstract given.