PDBsum entry 4p2b

Ligase

PDB id: 4p2b

Contents

Protein chain

507 a.a.

Ligands

SO4 ×2

PDB id:

4p2b

Name:

Ligase

Title:

Crystal structure of the apo form of the glutaminyl-tRNA synthetase catalytic domain from toxoplasma gondii.

Structure:

Glutamine aminoacyl-tRNA synthetase. Chain: a. Fragment: unp residues 332-886. Engineered: yes. Other_details: database gene sequence was optimized for codon usage before synthesis.

Source:

Toxoplasma gondii. Organism_taxid: 5811. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.80Å R-factor: 0.227 R-free: 0.256

Authors:

J.M.Van Rooyen,H.Belrhali,M.A.Hakimi

Key ref:

J.M.Van rooyen et al. Crystal structure of the apo form of the glutaminyl-T synthetase catalytic domain from toxoplasma gondii.. To be published, .

Date:

27-Feb-14 Release date: 18-Mar-15

Protein chain

B6KTZ0  (B6KTZ0_TOXGV) -  glutamine--tRNA ligase from Toxoplasma gondii (strain ATCC 50861 / VEG)

Seq: 860 a.a.

Struc: 507 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.6.1.1.18 - glutamine--tRNA ligase.
• Reaction: tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site