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PDBsum entry 4llr
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Oxidoreductase
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PDB id
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4llr
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PDB id:
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Oxidoreductase
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Title:
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Tryparedoxin peroxidase (txnpx) from trypanosoma cruzi in the reduced state
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Structure:
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Tryparedoxin peroxidase. Chain: a, b, c, d, e, f, g, h, i, j. Synonym: tryparedoxin peroxidase homologue. Engineered: yes
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Source:
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Trypanosoma cruzi. Organism_taxid: 5693. Strain: cl brener. Gene: tryp. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.80Å
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R-factor:
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0.164
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R-free:
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0.195
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Authors:
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M.D.Pineyro,J.C.Pizarro,F.Lema,O.Pritsch,A.Cayota,G.A.Bentley, C.Robello
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Key ref:
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M.D.Piñeyro
et al.
(2005).
Crystal structure of the tryparedoxin peroxidase from the human parasite Trypanosoma cruzi.
J Struct Biol,
150,
11-22.
PubMed id:
DOI:
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Date:
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09-Jul-13
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Release date:
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09-Oct-13
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Supersedes:
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PROCHECK
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Headers
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References
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O96763
(O96763_TRYCR) -
Putative tryparedoxin peroxidase from Trypanosoma cruzi
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Seq:
Struc:
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199 a.a.
194 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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DOI no:
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J Struct Biol
150:11-22
(2005)
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PubMed id:
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Crystal structure of the tryparedoxin peroxidase from the human parasite Trypanosoma cruzi.
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M.D.Piñeyro,
J.C.Pizarro,
F.Lema,
O.Pritsch,
A.Cayota,
G.A.Bentley,
C.Robello.
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ABSTRACT
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Tryparedoxin peroxidase from Trypanosoma cruzi (TcTXNPx) belongs to the family
of typical 2-Cys peroxiredoxins. These enzymes function as antioxidants through
their peroxidase and peroxynitrite reductase activities. In T. cruzi, as in all
trypanosomatids, this enzyme is the final electron acceptor of a unique system
for detoxifying hydroperoxides, constituting a relevant target for drug design.
We have determined the crystal structure of TcTXPNx in the reduced active state.
The structure comprises 10 subunits in the asymmetric unit, associated to form a
decamer of toroidal shape obeying 52 (D5) point group symmetry. We have analyzed
the structure of TcTXNPx by comparing it with other structures of typical 2-Cys
peroxiredoxins in both redox states, and have identified key residues in the
structural rearrangement taking place in the enzymatic cycle. This is the first
report of the structure of an active peroxiredoxin that has peroxidase and
peroxynitrite reductase activity, and it is noteworthy that it is from a human
parasite. This knowledge is of interest for further understanding peroxide
metabolism in these parasites, and in the design of new trypanosomatidal drugs
against Chagas disease.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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G.Colotti,
and
A.Ilari
(2011).
Polyamine metabolism in Leishmania: from arginine to trypanothione.
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Amino Acids,
40,
269-285.
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S.Patel,
S.Hussain,
R.Harris,
S.Sardiwal,
J.M.Kelly,
S.R.Wilkinson,
P.C.Driscoll,
and
S.Djordjevic
(2010).
Structural insights into the catalytic mechanism of Trypanosoma cruzi GPXI (glutathione peroxidase-like enzyme I).
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Biochem J,
425,
513-522.
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PDB code:
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F.B.Nogueira,
J.C.Ruiz,
C.Robello,
A.J.Romanha,
and
S.M.Murta
(2009).
Molecular characterization of cytosolic and mitochondrial tryparedoxin peroxidase in Trypanosoma cruzi populations susceptible and resistant to benznidazole.
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Parasitol Res,
104,
835-844.
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H.Castro,
and
A.M.Tomás
(2008).
Peroxidases of trypanosomatids.
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Antioxid Redox Signal,
10,
1593-1606.
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T.Jaeger,
and
L.Flohé
(2006).
The thiol-based redox networks of pathogens: unexploited targets in the search for new drugs.
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Biofactors,
27,
109-120.
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T.Nakamura,
T.Yamamoto,
T.Inoue,
H.Matsumura,
A.Kobayashi,
Y.Hagihara,
K.Uegaki,
M.Ataka,
Y.Kai,
and
K.Ishikawa
(2006).
Crystal structure of thioredoxin peroxidase from aerobic hyperthermophilic archaeon Aeropyrum pernix K1.
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Proteins,
62,
822-826.
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PDB code:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
