PDBsum entry 4k0r

Circadian clock protein

PDB id: 4k0r

Contents

Protein chain

463 a.a.

Waters ×71

PDB id:

4k0r

Name:

Circadian clock protein

Title:

Crystal structure of mouse cryptochrome 1

Structure:

Cryptochrome-1. Chain: a. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: cry1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108

Resolution:

2.65Å R-factor: 0.194 R-free: 0.260

Authors:

A.Czarna,E.Wolf

Key ref:

A.Czarna et al. (2013). Structures of Drosophila cryptochrome and mouse cryptochrome1 provide insight into circadian function. Cell, 153, 1394-1405. PubMed id: 23746849 DOI: 10.1016/j.cell.2013.05.011

Date:

04-Apr-13 Release date: 26-Jun-13

Protein chain

P97784  (CRY1_MOUSE) -  Cryptochrome-1 from Mus musculus

Seq: 606 a.a.

Struc: 463 a.a.

DOI no: 10.1016/j.cell.2013.05.011

Cell 153:1394-1405 (2013)

PubMed id: 23746849

Structures of Drosophila cryptochrome and mouse cryptochrome1 provide insight into circadian function.

A.Czarna, A.Berndt, H.R.Singh, A.Grudziecki, A.G.Ladurner, G.Timinszky, A.Kramer, E.Wolf.

ABSTRACT

Drosophila cryptochrome (dCRY) is a FAD-dependent circadian photoreceptor, whereas mammalian cryptochromes (CRY1/2) are integral clock components that repress mCLOCK/mBMAL1-dependent transcription. We report crystal structures of full-length dCRY, a dCRY loop deletion construct, and the photolyase homology region of mouse CRY1 (mCRY1). Our dCRY structures depict Phe534 of the regulatory tail in the same location as the photolesion in DNA-repairing photolyases and reveal that the sulfur loop and tail residue Cys523 plays key roles in the dCRY photoreaction. Our mCRY1 structure visualizes previously characterized mutations, an NLS, and MAPK and AMPK phosphorylation sites. We show that the FAD and antenna chromophore-binding regions, a predicted coiled-coil helix, the C-terminal lid, and charged surfaces are involved in FAD-independent mPER2 and FBXL3 binding and mCLOCK/mBMAL1 transcriptional repression. The structure of a mammalian cryptochrome1 protein may catalyze the development of CRY chemical probes and the design of therapeutic metabolic modulators.