PDBsum entry 4js7

Sugar binding protein

PDB id: 4js7

Contents

Protein chain

202 a.a.

Ligands

SO4

Waters ×292

PDB id:

4js7

Name:

Sugar binding protein

Title:

Crystal structure of d78n mutant apo form of clavibacter michiganensis expansin

Structure:

Cellulose binding protein. Chain: a. Fragment: unp residues 546-746. Engineered: yes. Mutation: yes

Source:

Clavibacter michiganensis subsp. Michiganensis. Organism_taxid: 443906. Strain: ncppb 382. Gene: cela, pcm1_0020. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.10Å R-factor: 0.155 R-free: 0.177

Authors:

N.H.Yennawar,H.P.Yennawar,N.Georgelis,D.J.Cosgrove

Key ref:

N.H.Yennawar et al. Crystal structure of wild type and d78n mutant clavib michiganensis expansin, In apo form and in complex wi oligosaccharides. To be published, .

Date:

22-Mar-13 Release date: 26-Mar-14

Protein chain

A5CLK3  (A5CLK3_CLAM3) -  cellulase from Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382)

Seq: 746 a.a.

Struc: 202 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.4 - cellulase.
• Reaction: Endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.