PDBsum entry 4h4s

Oxidoreductase

PDB id: 4h4s

Contents

Protein chain

401 a.a.

Ligands

GOL

FMT

FAD

Waters ×200

PDB id:

4h4s

Name:

Oxidoreductase

Title:

Crystal structure of ferredoxin reductase, bpha4 e175c/q177g mutant (reduced form)

Structure:

Biphenyl dioxygenase ferredoxin reductase subunit. Chain: a. Engineered: yes. Mutation: yes

Source:

Acidovorax. Organism_taxid: 358220. Strain: kks102. Gene: bpha4. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.65Å R-factor: 0.204 R-free: 0.225

Authors:

A.Nishizawa,A.Harada,M.Senda,Y.Tachihara,D.Muramatsu,S.Kishigami, S.Mori,K.Sugiyama,T.Senda,S.Kimura

Key ref:

A.Nishizawa et al. Random mutagenesis with the project assessment for co conversion of co-Factor specificity of a ferredoxin reductase bpha4. To be published, .

Date:

18-Sep-12 Release date: 09-Oct-13

Protein chain

E7FJB9  (E7FJB9_9BURK) -  Biphenyl dioxygenase ferredoxin reductase subunit from Acidovorax sp. KKS102

Seq: 408 a.a.

Struc: 401 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.18.1.3 - ferredoxin--NAD(+) reductase.
• Reaction:
  1. 2 reduced [2Fe-2S]-[ferredoxin] + NAD⁺ + H⁺ = 2 oxidized [2Fe- 2S]-[ferredoxin] + NADH
  2. reduced 2[4Fe-4S]-[ferredoxin] + NAD⁺ + H⁺ = oxidized 2[4Fe-4S]- [ferredoxin] + NADH
• Cofactor: FAD

FAD (Bound ligand (Het Group name = FAD) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site