PDBsum entry 4h2r

Oxidoreductase

PDB id: 4h2r

Contents

Protein chains

369 a.a.

Ligands

FAD ×2

BME

5HN

GOL ×5

Waters ×81

PDB id:

4h2r

Name:

Oxidoreductase

Title:

Structure of mhpco y270f mutant, 5-hydroxynicotinic acid complex

Structure:

2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Rhizobium loti. Mesorhizobium loti. Organism_taxid: 266835. Strain: maff303099. Gene: mlr6788. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.47Å R-factor: 0.210 R-free: 0.261

Authors:

J.Kobayashi,H.Yoshida,B.Mikami,H.Hayashi,S.Kamitori,T.Yagi

Key ref:

J.Kobayashi et al. Crystal structure of 2-Methyl-3-Hydroxypyridine-5-Car acid oxygenase. To be published, .

Date:

13-Sep-12 Release date: 18-Sep-13

Protein chains

Q988D3  (Q988D3_RHILO) -  2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

Seq: 379 a.a.

Struc: 369 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.14.12.4 - Transferred entry: 1.14.13.242.
• Reaction: 3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + O(2) = 2-(acetamidomethylene)succinate + NAD(P)(+)

3-hydroxy-2-methylpyridine-5-carboxylate (Bound ligand (Het Group name = 5HN) matches with 90.91% similarity) + NAD(P)H (Bound ligand (Het Group name = FAD) matches with 71.19% similarity) + O(2) = 2-(acetamidomethylene)succinate + NAD(P)(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site