PDBsum entry 4h0l

Photosynthesis

PDB id: 4h0l

Contents

Protein chains

213 a.a.

160 a.a.

286 a.a.

161 a.a.

32 a.a.

32 a.a.

37 a.a.

29 a.a.

Ligands

HEM ×4

OPC ×2

UMQ ×4

QNO

CLA

FES

SQD

BCR

Metals

_CD ×2

Waters ×6

PDB id:

4h0l

Name:

Photosynthesis

Title:

Cytochrome b6f complex crystal structure from mastigocladus laminosus with n-side inhibitor nqno

Structure:

Cytochrome b6. Chain: a. Cytochrome b6-f complex subunit 4. Chain: b. Synonym: 17 kda polypeptide. Apocytochrome f. Chain: c. Cytochrome b6-f complex iron-sulfur subunit. Chain: d.

Source:

Mastigocladus laminosus. Fischerella sp.. Organism_taxid: 83541. Organism_taxid: 83541

Resolution:

3.25Å R-factor: 0.220 R-free: 0.247

Authors:

S.S.Hasan,E.Yamashita,D.Baniulis,W.A.Cramer

Key ref:

S.S.Hasan et al. (2013). Quinone-dependent proton transfer pathways in the photosynthetic cytochrome b6f complex. Proc Natl Acad Sci U S A, 110, 4297-4302. PubMed id: 23440205 DOI: 10.1073/pnas.1222248110

Date:

08-Sep-12 Release date: 13-Feb-13

Protein chain

P83791  (CYB6_MASLA) -  Cytochrome b6 from Mastigocladus laminosus

Seq: 215 a.a.

Struc: 213 a.a.

Protein chain

P83792  (PETD_MASLA) -  Cytochrome b6-f complex subunit 4 from Mastigocladus laminosus

Seq: 160 a.a.

Struc: 160 a.a.

Protein chain

P83793  (CYF_MASLA) -  Cytochrome f from Mastigocladus laminosus

Seq: 333 a.a.

Struc: 286 a.a.*

Protein chain

P83794  (UCRI_MASLA) -  Cytochrome b6-f complex iron-sulfur subunit from Mastigocladus laminosus

Seq: 179 a.a.

Struc: 161 a.a.

Protein chain

P83795  (PETL_MASLA) -  Cytochrome b6-f complex subunit 6 from Mastigocladus laminosus

Seq: 32 a.a.

Struc: 32 a.a.

Protein chain

P83796  (PETM_MASLA) -  Cytochrome b6-f complex subunit 7 from Mastigocladus laminosus

Seq: 35 a.a.

Struc: 32 a.a.

Protein chain

P83797  (PETG_MASLA) -  Cytochrome b6-f complex subunit 5 from Mastigocladus laminosus

Seq: 37 a.a.

Struc: 37 a.a.

Protein chain

P83798  (PETN_MASLA) -  Cytochrome b6-f complex subunit 8 from Mastigocladus laminosus

Seq: 29 a.a.

Struc: 29 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain D: E.C.7.1.1.6 - plastoquinol--plastocyanin reductase.
• Reaction: 2 oxidized [plastocyanin] + a plastoquinol + 2 H⁺(in) = 2 reduced [plastocyanin] + a plastoquinone + 4 H⁺(out)

2 × oxidized [plastocyanin] (Bound ligand (Het Group name = QNO) matches with 50.00% similarity) + plastoquinol + 2 × H(+)(in) = 2 × reduced [plastocyanin] + plastoquinone + 4 × H(+)(out)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1073/pnas.1222248110

Proc Natl Acad Sci U S A 110:4297-4302 (2013)

PubMed id: 23440205

Quinone-dependent proton transfer pathways in the photosynthetic cytochrome b6f complex.

S.S.Hasan, E.Yamashita, D.Baniulis, W.A.Cramer.

ABSTRACT

No abstract given.