PDBsum entry 4g7s

Oxidoreductase

PDB id: 4g7s

Contents

Protein chains

554 a.a.

166 a.a.

31 a.a.

Ligands

HEM

HAS-PER

OLC ×15

CUA

Metals

_CU

Waters ×179

PDB id:

4g7s

Name:

Oxidoreductase

Title:

Structure of recombinant cytochrome ba3 oxidase mutant v236i from thermus thermophilus

Structure:

CytochromE C oxidase subunit 1. Chain: a. Synonym: cytochromE C ba(3) subunit i, cytochromE C oxidase polypeptide i, cytochrome cba3 subunit 1. Engineered: yes. Mutation: yes. CytochromE C oxidase subunit 2. Chain: b. Synonym: cytochromE C ba(3) subunit ii, cytochromE C oxidase

Source:

Thermus thermophilus. Organism_taxid: 300852. Strain: hb8 / atcc 27634 / dsm 579. Gene: cbaa, ttha1135. Expressed in: thermus thermophilus. Expression_system_taxid: 274. Gene: cbab, ctac, ttha1134. Gene: cbad, ttha1133.

Resolution:

2.00Å R-factor: 0.183 R-free: 0.207

Authors:

Y.Li,Y.Chen,C.D.Stout

Key ref:

Y.Li et al. Structure of recombinant cytochrome ba3 oxidase mutan from thermus thermophilus. To be published, .

Date:

20-Jul-12 Release date: 24-Jul-13

Protein chain

Q5SJ79  (COX1_THET8) -  Cytochrome c oxidase subunit 1 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 562 a.a.

Struc: 554 a.a.*

Protein chain

Q5SJ80  (COX2_THET8) -  Cytochrome c oxidase subunit 2 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 168 a.a.

Struc: 166 a.a.

Protein chain

P82543  (COXA_THET8) -  Cytochrome c oxidase polypeptide 2A from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 34 a.a.

Struc: 31 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C: E.C.7.1.1.9 - cytochrome-c oxidase.
• Reaction: 4 Fe(II)-[cytochrome c] + O2 + 8 H⁺(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H⁺(out)

4 × Fe(II)-[cytochrome c] + O2 + 8 × H(+)(in) (Bound ligand (Het Group name = PER) corresponds exactly) = 4 × Fe(III)-[cytochrome c] + 2 × H2O + 4 × H(+)(out)

• Cofactor: Cu cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site